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DOI :
10.3791/57519-v
May 10th, 2018
Chapters
0:04
Title
0:34
Preparation of mRNA
2:59
Toeprinting Assay
6:32
Sequencing Reactions and Gel Electrophoresis
7:53
Results: Importance of IRES Secondary Structure in the Formation of Translation Initiation Complexes on an Uncapped XIAP IRES RNA
9:35
Conclusion
Toeprinting 的目的是测量体外转录 RNA 的能力, 在各种条件下形成与核糖体的翻译起始配合物。该协议描述了一种 toeprinting 哺乳动物 RNA 的方法, 可用于研究 cap 依赖和 IRES 驱动的翻译。
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