Name: three-dimensional printing of thermoplastic materials to create automated syringe pumps with feedback control for microfluidic applications
Uploaded: 2018-08-30T12:30:00
Description: Watch this Scientific Journal Video about Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications at JoVE.com

Forfatterne bekrefter støtte fra Office of Naval Research awards N00014-17-12306 og N00014-15-1-2502, så vel som fra Luftforsvaret Office av vitenskapelig forskning prisbelønte FA9550-13-1-0108 og National Science Foundation Grant nr. 1709238.

1. 3D-utskrift og montering av sprøytepumpe
<ol><li>
Forberede og 3D-print sprøytepumpe komponenter
	<ol>
<li>Last ned den. STL design filer fra Tilleggsfiler til notatet. Merk: Det er seks. STL-filer, med tittelen 'JoVE_Syringe_Clamp_10mL_Size.stl', 'JoVE_Syringe_Platform.stl', 'JoVE_Syringe_Plunger_Connectors.stl', 'JoVE_Syringe_Pump_End_Stop.stl', 'JoVE_Syringe_Pump_Motor_Connector.stl', og ' JoVE_Syringe_Pump_Traveler_ Push.STL ", i tilleggsfiler. Disse filene...

<ol>
	<li>Sackmann, E. K., Fulton, A. L., Beebe, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+present+and+future+role+of+microfluidics+in+biomedical+research.">The present and future role of microfluidics in biomedical research.</a> Nature. 507 (7491), 181-189 (2014).</li><li>Duncombe, T. A., Tentori, A. M., Herr, A. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidics:+reframing+biological+enquiry.">Microfluidics: reframing biological enquiry.</a> Nature Reviews Molecular Cell Biology. 16 (9), 554-567 (2015).</li><li>Prakadan, S. M., Shalek, A. K., Weitz, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Scaling+by+shrinking:+empowering+single-cell+'omics'+with+microfluidic+devices.">Scaling by shrinking: empowering single-cell 'omics' with microfluidic devices.</a> Nature Reviews Genetics. 18 (6), (2017).</li><li>Kim, Y., Langer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidics+in+nanomedicine.">Microfluidics in nanomedicine.</a> Reviews in Cell Biology and Molecular Medicine. 1, 127-152 (2015).</li><li>Rusconi, R., Garren, M., Stocker, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidics+expanding+the+frontiers+of+microbial+ecology.">Microfluidics expanding the frontiers of microbial ecology.</a> Annual Review of Biophysics. 43, 65-91 (2014).</li><li>Skafte-Pedersen, P., Sabourin, D., Dufva, M., Snakenborg, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multi-channel+peristaltic+pump+for+microfluidic+applications+featuring+monolithic+PDMS+inlay.">Multi-channel peristaltic pump for microfluidic applications featuring monolithic PDMS inlay.</a> Lab on a Chip. 9 (20), 3003-3006 (2009).</li><li>Heo, Y. J., Kang, J., Kim, M. J., Chung, W. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tuning-free+controller+to+accurately+regulate+flow+rates+in+a+microfluidic+network.">Tuning-free controller to accurately regulate flow rates in a microfluidic network.</a> Scientific Reports. 6, 23273 (2016).</li><li>Kuczenski, B., LeDuc, P. R., Messner, W. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pressure-driven+spatiotemporal+control+of+the+laminar+flow+interface+in+a+microfluidic+network.">Pressure-driven spatiotemporal control of the laminar flow interface in a microfluidic network.</a> Lab on a Chip. 7 (5), 647-649 (2007).</li><li>Lake, J. R., Heyde, K. C., Ruder, W. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Low-cost+feedback-controlled+syringe+pressure+pumps+for+microfluidics+applications.">Low-cost feedback-controlled syringe pressure pumps for microfluidics applications.</a> PLoS One. 12 (4), (2017).</li><li>Kong, D. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Open-source,+community-driven+microfluidics+with+metafluidics.">Open-source, community-driven microfluidics with metafluidics.</a> Nature Biotechnology. 35 (6), 523-529 (2017).</li><li>Wijnen, B., Hunt, E. J., Anzalone, G. C., Pearce, J. 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Arduino Libraries for AccelStepper.h Available from: <a target="_blank" href="https://github.com/adafruit/AccelStepper">https://github.com/adafruit/AccelStepper</a> (2018)</li><li>Lin, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generation+of+dynamic+temporal+and+spatial+concentration+gradients+using+microfluidic+devices.">Generation of dynamic temporal and spatial concentration gradients using microfluidic devices.</a> Lab on a Chip. 4 (3), 164-167 (2004).</li><li>Korczyk, P. 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Her presentert vi en ny design for en sprøyte pumpesystem med lukket trykkontroll. Dette ble gjort ved å integrere en 3D-trykt sprøytepumpe med piezoresistive press sensor og en åpen kildekode mikrokontroller. Ved å bruke en PID-kontroller, var vi i stand til nettopp styre innløp trykket og gir raske svartider samtidig som stabiliteten om et angitt tidspunkt.
Mange eksperimenter med microfluidic enheter krever en presis fluidic kontroll og utnytte en godt karakterisert laminær strømnin...

Microfluidic verktøy har blitt nyttig for forskere i biologiske og kjemiske forskning. På grunn av lavt volum utnyttelse, rask måling funksjoner og veldefinert flyt profiler, microfluidics har fått trekkraft in genomisk og proteomic forskning, høy gjennomstrømming screening, medisinsk diagnostikk, nanoteknologi, og én celle analyse1,2,3,4. Videre fleksibiliteten til microfluidic konstruksjon gir enkel grunnleggende forskning, som undersøker spatiotemporal dynamikken i kulturperler bakteriell koloniene5.
Mange typer fluid innsprøytningen systemer er utviklet for å nøyaktig leverer strøm til microfluidic enheter. Eksempler på slike injeksjon systemer på en peristaltiske og resirkulering pumper6, trykk-kontrolleren systemer7og sprøyte pumper8. Disse injeksjon system, inkludert sprøyte pumper består ofte av dyre presisjonsnivå utviklet komponenter. Forsterke disse systemene med lukket tilbakemelding kontroll av trykket i produksjonen flyt legger kostnadene for disse systemene. Svar utviklet vi robuste, rimelige sprøyte pumpe systemer som bruker lukket tilbakemelding kontroll for å regulere outputted flyt press. Ved lukket trykkontroll er behovet for kostbare presisjon-utviklet komponenter avskaffet9.
Kombinasjonen av rimelig 3D-utskrift maskinvare og en betydelig vekst i tilhørende åpen kildekode programvare har gjort design og fabrikasjon av microfluidic enheter stadig tilgjengelig for forskere fra en rekke disipliner10. Imidlertid være den som brukes til å kjøre væske gjennom disse enhetene dyrt. For å løse dette behovet for en rimelig væske kontrollsystem, utviklet vi et design som kan være fabrikasjon av forskere i laboratoriet, krever bare et lite antall montering trinn. Til tross for sin lave kostnader og enkel montering, dette systemet kan gi nøyaktig flytkontroll og gir et alternativ til kommersielt tilgjengelige, lukket sprøyte pumpesystemer, som kan være uoverkommelig dyrt.
Her gir vi protokoller for bygging og bruk av closed-loop kontrollert sprøyte pumpesystem vi utviklet (figur 1). Væsken håndteringssystem består av en fysisk sprøytepumpe som er inspirert av en tidligere studie11, en microcontroller og et piezoresistive press sensor. Når samlet og programmert med en proporsjonal-integrert-derivat (PID) kontroller, er systemet i stand til å levere en velordnet, trykk-drevet flyt til microfluidic enheter. Dette gir et rimelig og fleksibelt alternativ til høye kostnader kommersielle produkter, som muliggjør en bredere gruppe forskere å bruke microfluidics i sitt arbeid.

<table border="0" cellpadding="0" cellspacing="0" width="1268">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:289px;">Arduino IDE</td>
			<td style="width:245px;">Arduino.org</td>
			<td style="width:169px;"></td>
			<td style="width:565px;">Arduino Uno R3 control software</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Header Connector, 2 Positions</td>
			<td>Digi-Key</td>
			<td>WM4000-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Header Connector, 3 Positions</td>
			<td>Digi-Key</td>
			<td>WM4001-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Header Connector, 4 Positions</td>
			<td>Digi-Key</td>
			<td>WM4002-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hook-up Wire, 22 Gauge, Black</td>
			<td>Digi-Key</td>
			<td>1528-1752-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hook-up Wire, 22 Gauge, Blue</td>
			<td>Digi-Key</td>
			<td>1528-1757-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hook-up Wire, 22 Gauge, Red</td>
			<td>Digi-Key</td>
			<td>1528-1750-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hook-up Wire, 22 Gauge, White</td>
			<td>Digi-Key</td>
			<td>1528-1768-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hook-up Wire, 22 Gauge, Yellow</td>
			<td>Digi-Key</td>
			<td>1528-1751-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Instrumentation Amplifier</td>
			<td>Texas Instruments</td>
			<td>INA122P</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Microcontroller, Arduino Uno R3</td>
			<td>Arduino.org</td>
			<td>A000066</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Mini Breadboard</td>
			<td>Amazon</td>
			<td>B01IMS0II0</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Power Supply</td>
			<td>BK Precision</td>
			<td>1550</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Pressure Sensor</td>
			<td>PendoTech</td>
			<td>PRESS-S-000</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Rectangular Connectors, Housings</td>
			<td>Digi-Key</td>
			<td>WM2802-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Rectangular Connectors, Male</td>
			<td>Digi-Key</td>
			<td>WM2565CT-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Resistors, 10k Ohm&#160;</td>
			<td>Digi-Key</td>
			<td>1135-1174-1-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Resistors, 330 Ohm&#160;</td>
			<td>Digi-Key</td>
			<td>330ADCT-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Stepper Motor Driver, EasyDriver</td>
			<td>Digi-Key</td>
			<td>1568-1108-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">USB 2.0 Cable, A-Male to B-Male</td>
			<td>Amazon</td>
			<td>PC045</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">3D Printed Material, Z-ABS&#160;</td>
			<td>Zortrax</td>
			<td></td>
			<td>A variety of colors are available</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">3D Printer</td>
			<td>Zortrax</td>
			<td>M200</td>
			<td>Printing out the syringe pump components</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Ball Bearing, 17x6x6mm</td>
			<td>Amazon</td>
			<td>B008X18NWK</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hex Machine Screws, M3x16mm&#160;</td>
			<td>Amazon</td>
			<td>B00W97MTII</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hex Machine Screws, M3x35mm&#160;</td>
			<td>Amazon</td>
			<td>B00W97N2UW</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hex Nut, M3 0.5&#160;</td>
			<td>Amazon</td>
			<td>B012U6PKMO</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Hex Nut, M5&#160;</td>
			<td>Amazon</td>
			<td>B012T3C8YQ</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Lathe Round Rod</td>
			<td>Amazon</td>
			<td>B00AUB73HW</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Linear Ball Bearing</td>
			<td>Amazon</td>
			<td>B01IDKG1WO</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Linear Flexible Coupler</td>
			<td>Amazon</td>
			<td>B010MZ8SQU</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Steel Lock Nut, M3 0.5</td>
			<td>Amazon</td>
			<td>B000NBKLOQ</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Stepper Motor, NEMA-17, 1.8o/step</td>
			<td>Digi-Key</td>
			<td>1568-1105-ND</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Syringe, 10mL, Luer-Lok Tip</td>
			<td>BD</td>
			<td>309604</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Threaded Rod</td>
			<td>Amazon</td>
			<td>B01MA5XREY</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">1H,1H,2H,2H-Perfluorooctyltrichlorosilane</td>
			<td>FisherScientific</td>
			<td>AAL1660609</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Camera Module</td>
			<td>Raspberry Pi Foundation</td>
			<td>V2</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Compact Oven</td>
			<td>FisherScientific</td>
			<td>PR305220G</td>
			<td>Baking PDMS pre-polymer mixture and the device</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dispensing Needle, 22 Gauge</td>
			<td>McMaster-Carr</td>
			<td>75165A682</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Dispensing Needle, 23 Gauge</td>
			<td>McMaster-Carr</td>
			<td>75165A684</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Fisherbrand Premium Cover Glasses</td>
			<td>FisherScientific</td>
			<td>12-548-5C</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Glass Culture Petri Dish, 130x25mm</td>
			<td>American Educational Products</td>
			<td>7-1500-5</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Plasma Cleaner</td>
			<td>Harrick Plasma</td>
			<td>PDC-32G</td>
			<td>Binding the cover glass with the PDMS device</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Razor Blades</td>
			<td>FisherScientific</td>
			<td>7071A141&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Scotch Magic Tape</td>
			<td>Amazon</td>
			<td>B00RB1YAL6</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Single-board Computer</td>
			<td>Raspberry Pi Foundation</td>
			<td>Raspberry Pi 2 model B</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Smart Spatula</td>
			<td>FisherScientific</td>
			<td>EW-06265-12</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Sylgard 184 Silicone Elastomer Kit</td>
			<td>FisherScientific</td>
			<td>NC9644388</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Syringe Filters</td>
			<td>Thermo Scientific</td>
			<td>7252520</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Tygon Tubing</td>
			<td>ColeParmer&#160;</td>
			<td>EW-06419-01</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Vacuum Desiccator</td>
			<td>FisherScientific</td>
			<td>08-594-15C</td>
			<td>Degasing PDMS pre-polymer mixture and coating fluorosilane on the master mold</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Weighing Dishes</td>
			<td>FisherScientific</td>
			<td>S67090A</td>
			<td></td>
		</tr>
	</tbody>
</table>

three-dimensional printing of thermoplastic materials to create automated syringe pumps with feedback control for microfluidic applications

MicroFluidics har blitt en betenkelig verktøyet i forskning på den biologiske, kjemiske og naturvitenskap. En viktig del av microfluidic eksperimentering er en stabil væske håndteringssystem kan nøyaktig gi en vik strømningshastighet eller innløp press. Her har vi utviklet en sprøyte pumpesystem kan styre og regulere innløp væsketrykk levert til en microfluidic enhet. Dette systemet ble utformet med rimelige materialer og additiv produksjon prinsipper, utnytte tredimensjonale (3D) utskrift av termoplastisk materiale og sokkel komponenter når det er mulig. Dette systemet består av tre hovedkomponenter: en sprøytepumpe og en trykktransduceren en programmerbar microcontroller. I dette papiret detalj vi et sett med protokoller for fabrikasjon, montering og programmering sprøyte pumpe systemet. Videre har vi inkludert representant resultater som viser høy-troskapen, tilbakemeldinger kontrollen innløp press med dette systemet. Vi forventer denne protokollen tillater forskere å dikte rimelig sprøyte pumpesystemer, redusere oppføring barrieren for bruk av microfluidics i biomedisinske, kjemiske, og materiale.

Her presenterer vi en protokoll for tilbakemelding-kontrollerte sprøyter pumpe systemet og viser potensiell bruk for microfluidic programmer. Figur 1 viser det tilkoblede systemet av sprøytepumpe, trykksensor, microfluidic enhet, microcontroller, press sensor krets og stepper motor driver. Detaljert bildeforklaringer for sprøyten pumpe montering er vist i figur 2 og elektronisk krets skjematisk for press sensing vises i <stron...

Watch this Scientific Journal Video about Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications at JoVE.com

Three-dimensional Printing of Thermoplastic Materials to Create Automated Syringe Pumps with Feedback Control for Microfluidic Applications

Her presenterer vi en protokoll for å konstruere en trykk-kontrollerte Sprøytepumpe brukes i microfluidic programmer. Denne sprøytepumpen er laget av en additively produserte kroppen, sokkel maskinvare og åpen kildekode elektronikk. Den resulterende systemet er rimelig, enkel å bygge, og leverer godt regulert strømning for å aktivere rask microfluidic forskning.

Tredimensjonale utskrift av termoplastisk materiale å lage automatiserte sprøyte pumper med tilbakemelding kontroll for Microfluidic applikasjoner

Microfluidics has become a critical tool in research across the biological, chemical, and physical sciences. One important component of microfluidic ...

Research

JoVE Journal

Bioengineering

Planar and Three-Dimensional Printing of Conductive Inks

Planar og tredimensjonale Trykking av Conductive blekk

Printed electronics rely on low-cost, large-area fabrication routes to create flexible or multidimensional electronic, optoelectronic, and biomedical ...

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Designing Silk-silke Protein legering materialer for Biomedical Applications

Fibrous proteins display different sequences and structures that have been used for various applications in biomedical fields such as biosensors, ...

Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology

Menneskelig bruskvev Fabrication Bruke Tredimensjonal Inkjet Printing Technology

Bioprinting, which is based on thermal inkjet printing, is one of the most attractive enabling technologies in the field of tissue engineering and ...

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Analyse av celle migrasjon innenfor en tre-dimensjonal Kollagen Matrix

The ability to migrate is a hallmark of various cell types and plays a crucial role in several physiological processes, including&#160;embryonic ...

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Generering av en Tredimensjonal Full Tykkelse hudekvivalent og Automated såret

In vitro models are a cost effective and ethical alternative to study cutaneous wound healing processes. Moreover, by using human cells, these models ...

Fabricating Superhydrophobic Polymeric Materials for Biomedical Applications

Fabrikere superhydrophobic polymere materialer for Biomedical Applications

Superhydrophobic materials, with surfaces possessing permanent or metastable non-wetted states, are of interest for a number of biomedical and industrial ...

Fabrication of Inverted Colloidal Crystal Poly(ethylene glycol) Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Fabrication of Inverted Colloidal Crystal Polyethylene glycol Scaffold: A Three-dimensional Cell Culture Platform for Liver Tissue Engineering

Fabrikasjon av invertert Kolloidalt Crystal Poly (etylenglykol) Stillas: En Tredimensjonal Cell Culture plattform for Liver Tissue Engineering

The ability to maintain hepatocyte function in vitro, for the purpose of testing xenobiotics&#39; cytotoxicity, studying virus infection and developing ...

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Fabrikasjon av Tredimensjonal Papirbaserte mikrofluid Enheter for immunologiske

Paper wicks fluids autonomously due to capillary action. By patterning paper with hydrophobic barriers, the transport of fluids can be controlled and ...

Melt Electrospinning Writing of Three-dimensional Poly(&#949;-caprolactone) Scaffolds with Controllable Morphologies for Tissue Engineering Applications

Melt Electrospinning Writing of Three-dimensional PolyÃƒÆ’Ã…Â½Ãƒâ€šÃ‚Âµ-caprolactone Scaffolds with Controllable Morphologies for Tissue Engineering Applications

Smelt Electrospinning skriving av tredimensjonale Poly(ε-caprolactone) stillaser med kontrollerbar Morphologies for Tissue Engineering programmer

This tutorial reflects on the fundamental principles and guidelines for electrospinning writing with polymer melts, an additive manufacturing technology ...

Antimicrobial Characterization of Advanced Materials for Bioengineering Applications

Antimikrobielle karakteristikk av avanserte materialer for bioteknologi programmer

The development of new advanced materials with enhanced properties is becoming more and more important in a wide range of bioengineering applications. ...