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DOI :
10.3791/57547-v
April 16th, 2018
Chapters
0:04
Title
1:10
Purification of PDGFRα Positive Oligodendrocyte Lineage Cells from Mouse
5:03
Low-cell ChIP Preparation and ChIP Library Construction for High-throughput Sequencing
9:41
Results: Use of ChIP Analysis to Study Genome-wide Binding of the Olig2 in OPCs
11:42
Conclusion
在这里, 我们提出一个协议, 旨在分析少突胶质转录因子 2 (Olig2) 在急性纯化脑少突胶质前体细胞 (OPCs) 的全基因组结合执行低细胞染色质免疫沉淀 (芯片)、库准备、高通量测序和生物信息学数据分析。
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