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DOI :
10.3791/57593-v
May 2nd, 2018
Chapters
0:04
Title
0:50
Preparation for Ovary Dissection and Culture
2:13
Ovary Dissection and Organ Culture
3:34
Tissue Fixation
4:12
Whole Mount Immunofluorescence
5:42
Ovary Clearing for Imaging
6:37
Results: Immunofluorescence Imaging of Cleared Ovaries
7:23
Conclusion
在这里, 我们提出一项协议, 以量化卵泡在培养卵巢小鼠没有连续切片。用全器官免疫荧光和组织清除, 用光学切片代替物理切片。这种样品制备和可视化方法保持了器官的完整性, 促进了特定细胞的自动定量。
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