Name: a novel method for the pentosan analysis present in jute biomass and its conversion into sugar monomers using acidic ionic liquid
Uploaded: 2018-06-01T14:30:00
Description: Watch this Scientific Journal Video about A Novel Method for the Pentosan Analysis Present in Jute Biomass and Its Conversion into Sugar Monomers Using Acidic Ionic Liquid at JoVE.com

我们要感谢台湾科技部 (104-2628-E-002-008-MY3; 105-2218-155-007; 105-2221-e-002-003-MY3; 105-2221-e-002-227-MY3; 105-2622-e-155-003-CC2) 与国立台湾顶尖大学项目的目标大学 (105R7706) 为资金支持。我们感谢世界银行通过一项高等教育质量提高项目 (HEQEP) 的分项目, #2071 的完整建议, 为这项工作提供部分资金。这项工作也得到了卧龙岗大学 AIIM (金基金) 的部分支持。

在呈现的工作中使用的几种化学物质是有毒和致癌的。在执行 IL 和生物量处理的合成时, 请使用所有适当的安全做法。
1. 酸性 IL 的制备
<ol><li>在50毫升的圆底烧瓶中加入7.625 毫摩尔 13-丙烷 sultone, 然后用橡胶隔膜关闭烧瓶。</li>
	<li>使用注射器 (10 毫升) 在0°c 添加7.625 毫摩尔 1-甲基咪唑到7.625 毫摩尔 13-propanesultone 缓慢 (1 分钟)。</li>
	<li>在完全添加 1-甲基咪唑和 13-propanesultone...

<ol>
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Technol. 169 (Supplement C), 336-343 (2014).</li><li>Shill, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ionic+liquid+pretreatment+of+cellulosic+biomass:+Enzymatic+hydrolysis+and+ionic+liquid+recycle.">Ionic liquid pretreatment of cellulosic biomass: Enzymatic hydrolysis and ionic liquid recycle.</a> Biotechnol Bioeng. 108 (3), 511-520 (2011).</li><li>Tadesse, H., Luque, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Advances+on+biomass+pretreatment+using+ionic+liquids:+An+overview.">Advances on biomass pretreatment using ionic liquids: An overview.</a> Energy Environ. Sci. 4 (10), 3913-3929 (2011).</li><li>Agirrezabal-Telleria, I., Gandarias, I., Arias, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Production+of+furfural+from+pentosan-rich+biomass:+Analysis+of+process+parameters+during+simultaneous+furfural+stripping.">Production of furfural from pentosan-rich biomass: Analysis of process parameters during simultaneous furfural stripping.</a> Bioresour. Technol. 143 (Supplement C), 258-264 (2013).</li><li>Yingying, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+Improved+Method+for+Determination+of+Pentosans+in+Pulps+using+Dual-Wavelength+Spectroscopy.">An Improved Method for Determination of Pentosans in Pulps using Dual-Wavelength Spectroscopy.</a> BioResources. 11 (3), 6801-6807 (2016).</li><li>Kumar, A. K., Sharma, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+updates+on+different+methods+of+pretreatment+of+lignocellulosic+feedstocks:+a+review.">Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review.</a> Bioresour. Bioprocess. 4 (1), 7 (2017).</li><li>Kumar, P., Barrett, D. M., Delwiche, M. J., Stroeve, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methods+for+Pretreatment+of+Lignocellulosic+Biomass+for+Efficient+Hydrolysis+and+Biofuel+Production.">Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production.</a> Ind. Eng. Chem. Res. 48 (8), 3713-3729 (2009).</li></ol>

将黄麻生物量转化为 C5 糖单体的戊聚糖表现为使用各种均质 Brønsted 酸性催化剂, 如H2,因此4、HCl 和酸性 IL。并将酸性 il 的催化效果与无酸度的 il (1-丁基 3-咪唑氯化氯) 进行了比较。所有反应都是在160摄氏度的巴里高压釜中进行的。酸性 IL 的使用表明, 与这项工作中使用的均质酸 (矿物酸 H2, 所以4和 HCl) 相比, 戊聚糖转化率最高。结果表明, 酸性 IL 具有较高的 C5 糖?...

生物量作为可再生能源和化学来源具有很大的潜力, 因为它是可持续的、廉价的、同样分布不像化石资源, 这使得它成为替代化石原料的最有希望的候选国之一。纤维素生物量的估计产量为每年1460亿公吨1。纤维素生物量主要由木质素、纤维素和半纤维素组成, 为其三主要成分。木质素是由 phenylpropanoid 单位制成的芳香聚合物;另一方面, 纤维素和半纤维素是纤维素生物量的多糖部分。纤维素由β (1→4) 糖苷链连接的葡萄糖单元组成, 而半纤维素由β (1→4)、β (1→3) 和β (1→6) 糖苷键2、3组成, 由 C5 糖、C6 糖和糖酸共同构成。与各种纤维素生物量 (蔗渣, 稻壳, 麦草,等), 黄麻木质纤维素的生物量也产生了很大数量 (ca. 98% 2014年) 在亚洲与世界黄麻总产量相比。印度生产 1.96 x 106公吨黄麻生物量, 而孟加拉国生产 1.34 x 106公吨的黄麻生物量, 与世界黄麻生物量总产量 (3.39 x 106公吨) 相比, 在 2014年4。这种非食用生物量的利用不会与粮食需求发生冲突。因此, 将其用作合成各种增值化学品 (木糖、阿拉伯糖、糠醛、5-hydroxymethylfurfural (HMF)、等) 的原料是有益的. 根据美国能源部的数据, 糠醛和 HMF 被认为是来自生物量5的前30块建筑化学物质中的一些。糠醛是从木糖或直接从半纤维素得到的, 可以转化为许多重要的化学物质。糠醇、甲基呋喃和四氢呋喃是从糠醛6中获得的重要化学物质。因此, 将纤维素生物量 (如黄麻生物量) 转化为 C5 糖和其他重要化学物质是一个重要的课题。
关于将纤维素生物量转化为增值化学品的各种催化方法, 可提供广泛的报告。矿物酸 (HCl 和 H2因此4) 和异质催化剂 (Amberlyst、HMOR、HUSY、SAPO-44、等) 被大量用于将半纤维素和纤维素生物量转化为糖 (戊和己糖依赖糖)和呋喃 (糠醛和 HMF)7,8。矿物酸的复用性和腐蚀是一个重要问题。然而, 随着固体酸催化剂的出现, 由于反应发生在催化剂表面, 因此需要较高的温度和压力。为了克服这些问题, 最近报告了测算的生物量作为催化剂或溶剂9,10,11,12,13,14。使用 IL 作为溶剂不是一个更好的方法, 因为它的成本较高, 并降低蒸汽压力的 ILs, 造成了困难的产品分离。因此, 必须在水溶剂系统中使用可回收 IL 作为催化剂 (少量), 以便将生物质转化为增值化学品。
在这里, 我们提出了使用 1-甲基 3-(3-磺丙基) 鎓硫酸氢酸性 IL 作为催化剂, 以直接转化为戊聚糖在黄麻生物量成糖单体, 没有任何预处理。通常, 纤维素生物量10、15、16、17的预处理报告了 ils, 而大量的 ils 用于生物量预处理。因此, 使用 IL 作为催化剂并将纤维素生物量转化为化学物质, 而无需任何额外的处理, 总是有利的。此外, 在目前的工作中, 用 Klason 方法计算了黄麻生物量中的木质素浓度, 可转化为各种芳香单体18。

<table>
	<tbody>
		<tr>
			<td>1-Methylimidazole</td>
			<td>Sigma Aldrich</td>
			<td>M50834</td>
			<td></td>
		</tr>
		<tr>
			<td>1,3-Propanesultone</td>
			<td>Sigma Aldrich</td>
			<td>P50706</td>
			<td>Moisture sensitive</td>
		</tr>
		<tr>
			<td>p-nitroaniline</td>
			<td>Sigma Aldrich</td>
			<td>185310</td>
			<td></td>
		</tr>
		<tr>
			<td>Toluene</td>
			<td>J. T. Baker</td>
			<td>9460-03</td>
			<td></td>
		</tr>
		<tr>
			<td>Sulfuric acid</td>
			<td>Honeywell-Fluka</td>
			<td>30743</td>
			<td>Highly corrosive</td>
		</tr>
		<tr>
			<td>Hydrochloric acid</td>
			<td>Honeywell-Fluka</td>
			<td>30719</td>
			<td>Highly corrosive</td>
		</tr>
		<tr>
			<td>1-butyl-3-methylimidazolium chloride</td>
			<td>Sigma Aldrich</td>
			<td>900856</td>
			<td>Highly hygroscopic</td>
		</tr>
		<tr>
			<td>D(+)-Xylose</td>
			<td>Acros Organics</td>
			<td>141001000</td>
			<td></td>
		</tr>
		<tr>
			<td>L(+)-Arabinose</td>
			<td>Acros Organics</td>
			<td>104981000</td>
			<td></td>
		</tr>
		<tr>
			<td>UV-Spectrometer</td>
			<td>JASCO</td>
			<td>V-670</td>
			<td></td>
		</tr>
		<tr>
			<td>Parr reactor</td>
			<td>Parr USA</td>
			<td>Seriese 4560</td>
			<td></td>
		</tr>
		<tr>
			<td>Parr reactor controller</td>
			<td>Parr USA</td>
			<td>Seriese 4848</td>
			<td></td>
		</tr>
		<tr>
			<td>High pressure liquid chromatography (HPLC)</td>
			<td>JASCO</td>
			<td>Seriese LC-2000</td>
			<td></td>
		</tr>
		<tr>
			<td>Digital hot plate stirrer</td>
			<td>Thermo Scientific</td>
			<td>SP142020-33Q Cimarec</td>
			<td></td>
		</tr>
		<tr>
			<td>Oven furnace</td>
			<td>Thermal Scientific</td>
			<td>FB1400 Thermolyne blast oven furnace</td>
			<td></td>
		</tr>
	</tbody>
</table>

a novel method for the pentosan analysis present in jute biomass and its conversion into sugar monomers using acidic ionic liquid

近年来, 离子液体 (ILs) 被用于生物质测算, 因为它们具有热稳定性、较低的蒸气压、不可燃性、较高的热容量、可调谐的溶解度和酸度等显著特性。通过利用 Brønsted 酸性 1-甲基-3-(3-磺丙基)-鎓硫酸氢的催化量, 我们演示了在一锅工艺中从黄麻生物量的戊聚糖中合成 C5 糖 (木糖和阿拉伯糖) 的方法。酸性 IL 是在实验室合成的, 其特点是使用核磁共振光谱技术来了解其纯度。测定了不同的保水性能, 如酸强度、热、热液稳定性等, 表明催化剂在较高温度下稳定 (250 摄氏度), 具有很高的耐酸强度 (Ho 1.57)。酸性 IL 将超过90% 的戊聚糖转化为糖和糠醛。因此, 本研究提出的方法也可用于其他纤维素生物量戊聚糖浓度的评价。

从生物量中回收的戊聚糖和木质素的确切数量取决于纤维素生物量的类型。从不同的地方收集的纤维素生物量的相似类型可以有不同浓度的戊聚糖和木质素。本研究中使用的黄麻生物量包含20个戊聚糖% 和 14 个小波% 木质素。
图 1显示了矿物酸 (H2、4和 HCl) 和酸性 IL 对黄麻生?...

Watch this Scientific Journal Video about A Novel Method for the Pentosan Analysis Present in Jute Biomass and Its Conversion into Sugar Monomers Using Acidic Ionic Liquid at JoVE.com

A Novel Method for the Pentosan Analysis Present in Jute Biomass and Its Conversion into Sugar Monomers Using Acidic Ionic Liquid

我们提出了一个协议, 以合成 C5 糖 (木糖和阿拉伯糖) 从可再生的不可食用的纤维素生物量 (即, 黄麻) 与存在 Brønsted 酸性离子液体 (捞) 作为催化剂在水中.该催化剂比传统的矿物酸催化剂具有更好的催化性能 (H2, 因此4和 HCl)。

利用酸性离子液体戊聚糖分析黄麻生物量及其转化为糖单体的新方法

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The whitefly Bemisia tabaci (Gennadius) is an invasive pest of considerable importance, affecting the production of vegetable and ornamental crops in many ...

An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings

An Induction System for Clustered Stomata by Sugar Solution Immersion Treatment in Arabidopsis thaliana Seedlings

拟南芥幼苗糖溶液浸入治疗的聚集性植物诱导系统

Stomatal movement mediates plant gas exchange, which is essential for photosynthesis and transpiration. Stomatal opening and closing are accomplished by a ...

Microalgae Cultivation and Biomass Quantification in a Bench-Scale Photobioreactor with Corrosive Flue Gases

具有腐蚀性烟气的台式光生物反应器中的微藻培养和生物量定量

Photobioreactors are illuminated cultivation systems for experiments on phototrophic microorganisms. These systems provide a sterile environment for ...