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DOI :
10.3791/57629-v
August 15th, 2018
Chapters
0:04
Title
0:33
Dissection of the Intestinal Segment
3:01
Removal of the Epithelial Mucosa
4:57
Collection of Enteric Glial Cells from the Lamina Propria and Submucosa
6:23
Results: Enteric Glial Cells 24 h After Plating
7:12
Conclusion
在这里, 我们描述了肠道黏膜下层的肠神经胶质细胞的分离, 使用顺序 EDTA 孵化螯合价阳离子, 然后孵化在非酶细胞恢复解决方案。在聚 d-赖氨酸和层粘连蛋白的作用下, 电镀细胞悬浮物会导致黏膜下胶质细胞的高度富集培养, 用于功能分析。
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