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DOI :
10.3791/57643-v
•
12:52 min
May 12th, 2018
Chapters
0:05
Title
0:50
Treatment of Cells, Transfection, and Seeding onto Coverslips
2:54
Assembly of Microscopy Imaging Chamber
3:43
Microinjection Procedure
5:02
FRAP Procedure
5:32
Photoactivation Procedure
6:26
Data Analysis
9:42
Results: Analysis of Morphogenetic Dynamics and Turnover of Cytoskeletal Regulators
11:27
Conclusion
我们描述了微和 photomanipulation 技术, 如酶和 photoactivation 如何能够确定运动参数和蛋白质在迁移细胞内的时空动力学。实验读数包括亚细胞动力学和运动调节器的更替或底层肌动蛋白骨架。
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