膜分馏和 gtpase-关联免疫吸附法检测小 gtpase 预基化和 gtp 结合
November 11th, 2018
•Javad Alizadeh1,2, Shahla Shojaei1,2,3, Simone da Silva Rosa1, Adel Rezaei Moghadam1,2, Amir A. Zeki4, Mohammad Hashemi5, Marek J. Los6,7,8, Joseph W. Gordon1,9, Saeid Ghavami1,2,10
1Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, 2Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, 3Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 4Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, 5Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, 6Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, 7Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, 8LinkoCare Life Sciences AB, 9College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, 10Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences
在这里, 我们描述了一个方案, 以研究前化和鸟苷-5 ' 三磷酸 (gtp) 加载的 rho gtpase。该方案由两种详细的方法组成, 即膜分馏和 gtpase 连锁免疫吸附法。该协议可用于测量不同其他小型 gtpase 的预化和 gtp 负载。
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