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DOI :
10.3791/57687-v
June 3rd, 2018
Chapters
0:05
Title
0:46
Production of Retrovirus and Transduction of MEFs
5:01
Immunostaining of Reprogrammed MEFs
6:44
Results: Over-expression of GHMT2m and Inhibition of TGF-β Signaling Increases the Reprogramming Efficiency of MEFs into Functional Cardiomyocytes
8:34
Conclusion
通过 GATA4、Hand2、Mef2c、Tbx5、miR-1 和 miR-133 (GHMT2m) 的过度表达和 TGF β信号的抑制, 我们提出了一种健壮的方法, 将原发成纤维细胞重新编程成功能性心肌细胞。我们的协议在7天后的转导中产生跳动的心肌细胞, 效率高达60%。
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