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DOI :
10.3791/57690-v
June 30th, 2018
Chapters
0:04
Title
0:54
Physical Alignment of Image Splitter Generated Images
2:25
Alignment of Spectrally Separated Channels by Software Performing Spatial Transformation
4:34
Single Molecule Imaging of Mitochondrial Membrane Proteins
8:32
Results: Multi-color Localization of Mammalian Mitochondrial PINK1
10:15
Conclusion
여기, 우리 살아있는 세포의 세포에 단일 막 단백질의 다 색 지역화에 대 한 프로토콜을 제시. Fluorophores 연결할 자체 라벨 단백질 사용 됩니다. 단백질, 세포 기관이 동일한의 다른 막 구획에 있는 ~ 18의 정밀도로 지역화할 수 nm.
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