Development of a Multispecies Community Representative of the Oral Microbiome
2:12
Maintenance and Passaging of Caco-2 and HT29-MTX Cell Lines
3:20
Assembly of Multicompartment Cell Model Simulating the Gut Host-microbe Interface
5:27
Bacterial Survival Following In Vitro Gastrointestinal Transit Conditions
6:50
Bacterial Colonization Ability Following In Vitro Gastrointestinal Transit Conditions
8:13
Results: Viability of Synthetic Bacterial Community on In Vitro Gut Host Microbe System
9:31
Conclusion
Transcript
This model is the next step in setting host microdirections for the upper digestive tracts. It is allowing addressing mechanistic research questions and it offers many opportunities for the food, the pharma, and nutraceutical industry and also tox
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Gut host-microbe interactions were assessed using a novel approach combining a synthetic oral community, in vitro gastrointestinal digestion, and a model of the small intestine epithelium. We present a method that can be adapted to evaluate cell invasion of pathogens and multi-species biofilms, or even to test probiotic formulations' survivability.