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DOI :
10.3791/57712-v
May 27th, 2018
Chapters
0:05
Title
0:41
Expression of Qβ
2:51
Purification of Qβ
5:51
Conjugating DB Compounds on Qβ
8:06
Results: Qβ-PEG Can Be Used as a Fluorescence Probe Inside Cells
9:35
Conclusion
在这里, 我们提出一个程序, 荧光 functionalize 二硫化物 Qβ vip 与 dibromomaleimide。我们描述了 Qβ的表达和纯化, dibromomaleimide 功能分子的合成, dibromomaleimide 和 Qβ的共轭反应。生成的黄色荧光共轭粒子可作为细胞内的荧光探针。
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