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DOI :
10.3791/57716-v
June 13th, 2018
Chapters
0:04
Title
0:45
Tissue Preparation and Developmental Staging of Drosophila melanogaster Pupa
2:24
Dissection of D. melanogasterAntennal Discs at 0 - 8 h APF
4:17
Dissection of D. melanogaster Mid-pupal Antennae
5:43
Dissection of D. melanogaster Adult Antennae
7:02
Results: Analyses of Prepared Tissues
8:02
Conclusion
在这里, 我们提出了一个阶段和解剖发展的嗅觉组织从果蝇物种的协议。解剖后的组织可用于分子分析, 如定量 rt-pcr (反向转录-聚合酶链反应) 或 RNA 测序 (RNAseq), 以及体内分析, 如免疫组化或原位杂交。
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