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DOI :
10.3791/57716-v
June 13th, 2018
Chapters
0:04
Title
0:45
Tissue Preparation and Developmental Staging of Drosophila melanogaster Pupa
2:24
Dissection of D. melanogasterAntennal Discs at 0 - 8 h APF
4:17
Dissection of D. melanogaster Mid-pupal Antennae
5:43
Dissection of D. melanogaster Adult Antennae
7:02
Results: Analyses of Prepared Tissues
8:02
Conclusion
ここでは、ステージし、解剖ショウジョウバエ種から嗅覚の組織を開発するためのプロトコルを提案する.解剖組織は、免疫組織化学または上皮内などの生体内解析だけでなく、定量的 RT-PCR (逆転写ポリメラーゼ連鎖反応) や RNA 配列 (RNAseq) などの分子生物学的解析の後で使用できます。交配。
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