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DOI :
10.3791/57720-v
•
8:23 min
August 6th, 2018
Chapters
0:04
Title
0:47
Preparation of Plant Cell Material
1:55
Tandem Affinity Purification (TAP)
4:59
Metabolite and Protein Extraction
6:15
Results: Simultaneous Characterization of Protein-Protein and Protein-Metabolite Interactions Employing a Single-Step Tandem Affinity Purification Coupled with Mass Spectrometry
7:23
Conclusion
蛋白质和蛋白质代谢物相互作用对所有细胞功能都至关重要。在这里, 我们描述了一个协议, 允许并行分析这些交互与蛋白质的选择。我们的协议优化了植物细胞培养, 并结合亲和纯化与质谱为基础的蛋白质和代谢物检测。
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