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DOI :
10.3791/57724-v
May 31st, 2018
Chapters
0:04
Title
0:51
Respirometer and Colorimeter Preparation
2:25
Human Hepatocellular Carcinoma (HepG2) Cell Preparation
4:26
Calorimetry and Respirometry
6:36
Results: Representative O2 Concentration and Flux Analyses
8:23
Conclusion
このプロトコルでは、calorespirometry、放熱の呼吸、エネルギー代謝を評価するために非侵襲的アプローチを提供する直接・同時測定をについて説明します。この手法は、総細胞エネルギーの流れを監視することによって好気性と嫌気性経路エネルギー利用への貢献を評価するために使用されます。
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