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JoVE Journal

Biochemistry

This content is Open Access.

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

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08:58 min

July 5th, 2018

July 5th, 2018

12,379 Views

0:05

Title

0:38

Purification of Soluble Glycoprotein from HEK293 Supernatant

2:08

Crystallization of Glycoproteins

3:44

Soaking Glycoprotein Crystals with its Ligand

4:33

Characterization of Fab and Small Molecule Binding to the Glycoprotein

7:34

Results: Characterization of Glycoproteins

8:24

Conclusion

Transcript

We present approaches for the biophysical and structural characterization of glycoproteins with the immunoglobulin fold by biolayer interferometry, isothermal titration calorimetry, and X-ray crystallography.

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