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DOI :
10.3791/57765-v
May 24th, 2018
Chapters
0:04
Title
0:42
Larval Glue Dissection
2:36
Electrical Stimulation: FM Dye Loading and Unloading
4:08
Channelrhodopsin Stimulation: FM Dye Loading and Unloading
5:21
Fluorescence Quantification
6:12
Results: Synaptic Ultrastructure with FM Dye Photoconversion to Mark Vesicles
7:22
Conclusion
突触囊泡 (SV) 循环是神经元突触中细胞间通讯的核心机制。调频染料的摄取和释放是定量测定 SV 内胞吐作用的主要手段。在这里, 我们比较所有的刺激方法, 以驱动 FM1-43 循环在果蝇神经肌肉连接 (NMJ) 模型突触。
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