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DOI :
10.3791/57772-v
May 20th, 2018
Chapters
0:05
Title
0:35
Restriction Enzyme Treatment, Bisulfite Conversion, and Design of Bisulfite Sequencing Primers
1:54
Bisulfite Converted PCR and Gel Extraction
4:16
Bisulfite Sequencing Library Preparation
10:44
Results: Accurate Quantification of mtDNA Methylation
11:48
Conclusion
在这里, 我们提出了一个协议, 允许准确定量的线粒体 DNA (线粒体) 甲基化。在本协议中, 我们描述了一种酶消化 DNA 与BamHI结合的生物信息学分析管道, 可用于避免高估线粒体甲基化水平引起的线粒体二级结构。
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