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DOI :
10.3791/57776-v
September 25th, 2018
Chapters
0:04
Title
0:46
Gene Expression Assay Preparation and Surface Staining of Viable Cells
3:18
Prepare Cell Collection Plates, Perform FACS Sort, and Generate cDNA
5:17
Multiplex qPCR on the Biomark Platform
11:26
Results: Single-cell Quantitation of mRNA and Surface Protein Expression
12:56
Conclusion
96 遺伝子の発現を量的に方法論が説明し、単一セルがex vivo、差動の同定を可能にする 18 の表面タンパク質は感染していない細胞を基準にしてウイルスに感染した細胞の遺伝子そして蛋白質を表明しました。+ T 研究 SIV 感染 CD4 にアプローチを適用アカゲザルから分離した細胞。
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