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DOI :
10.3791/57791-v
August 24th, 2018
Chapters
0:04
Title
0:41
Differentiation of Human Pluripotent Stem Cells (hPSC) to Retinal Pigment Epithelium (RPE) Cells and Isolation
3:02
Whole-cell Patch Clamp RPE Cell Culture Preparation
4:42
Results: Representative Mature RPE Cell Status Validation
5:28
Conclusion
患者由来の変異ひと多能性幹細胞からの網膜色素上皮 (RPE) を区別するためにプロトコルをご紹介します。変異細胞は、免疫ブロット、蛍光抗体法、パッチク ランプなど機能の分析に使用可能性があります。この病気の料理方法は、ネイティブのひと網膜色素上皮細胞を得ることが困難を回避できます。
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