Name: rapid, scalable assembly and loading of bioactive proteins and immunostimulants into diverse synthetic nanocarriers via flash nanoprecipitation
Uploaded: 2018-08-11T14:00:00
Description: Watch this Scientific Journal Video about Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation at JoVE.com

Erkjenner vi ansatte og instrumentering støtte fra strukturell biologi anlegget ved Northwestern University. Støtten fra det R.H. Lurie omfattende Cancer Center av Northwestern universitetet og Northwestern University strukturell biologi fasilitetene er anerkjent. Et K2 direkte elektron detektoren ble kjøpt med midler gitt av Chicago biomedisinsk Consortium med støtte fra Searle midlene på The Chicago samfunnet Trust. Vi takker også følgende fasiliteter ved Northwestern University: Keck tverrfaglig overflaten Science anlegget, strukturell biologi anlegget, biologiske Imaging anlegget, Center for avansert molekylær Imaging og Analytical Bionanotechnology utstyr kjerne. Denne forskningen ble støttet av National Science Foundation grant 1453576, nasjonale institutter for helse direktør nye innovatør Award 1DP2HL132390-01, Center for regenerativ Nanomedicine Catalyst Award og 2014 McCormick katalysator Award. SDA var støttes delvis av NIH predoctoral bioteknologi trening Grant T32GM008449.

1. syntese av Poly(ethylene glycol) -blokk-poly (propylen sulfide)-Thiol
<ol><li>Syntetisere metoksy-poly(ethylene glycol) mesylate (Mn: 750) (MeO-pinne17- MULTIPLE SCLEROSIS, jeg).<ol>
<li>Oppløse 10 g MeO-pinne17-OH i 200 mL av toluen 100% innen en 3-hals runde bunnen kolbe (RBF) under magnetiske omrøring på 600 rpm.</li>
		<li>3-hals RBF tilkobles en Dean-Stark apparater, selv knyttet til en kondensator, holde hele systemet under dekkgass nitrogen eller argon.</li>
...

<ol>
	<li>Stano, A., Scott, E. A., Dane, K. Y., Swartz, M. A., Hubbell, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tunable+T+cell+immunity+towards+a+protein+antigen+using+polymersomes+vs.+solid-core+nanoparticles.">Tunable T cell immunity towards a protein antigen using polymersomes vs. solid-core nanoparticles.</a> Biomaterials. 34 (17), 4339-4346 (2013).</li><li>Discher, B. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polymersomes:+tough+vesicles+made+from+diblock+copolymers.">Polymersomes: tough vesicles made from diblock copolymers.</a> Science. 284 (5417), 1143-1146 (1999).</li><li>Vasdekis, A. E., Scott, E. A., O'Neil, C. P., Psaltis, D., Hubbell, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Precision+intracellular+delivery+based+on+optofluidic+polymersome+rupture.">Precision intracellular delivery based on optofluidic polymersome rupture.</a> ACS Nano. 6 (9), 7850-7857 (2012).</li><li>Yi, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tailoring+Nanostructure+Morphology+for+Enhanced+Targeting+of+Dendritic+Cells+in+Atherosclerosis.">Tailoring Nanostructure Morphology for Enhanced Targeting of Dendritic Cells in Atherosclerosis.</a> ACS Nano. 10 (12), 11290-11303 (2016).</li><li>Shum, H. C., Kim, J. W., Weitz, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidic+fabrication+of+monodisperse+biocompatible+and+biodegradable+polymersomes+with+controlled+permeability.">Microfluidic fabrication of monodisperse biocompatible and biodegradable polymersomes with controlled permeability.</a> Journal of the American Chemical Society. 130 (29), 9543-9549 (2008).</li><li>Pessi, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidics-assisted+engineering+of+polymeric+microcapsules+with+high+encapsulation+efficiency+for+protein+drug+delivery.">Microfluidics-assisted engineering of polymeric microcapsules with high encapsulation efficiency for protein drug delivery.</a> International Journal of Pharmaceutics. 472 (1-2), 82-87 (2014).</li><li>O'Neil, C. P., Suzuki, T., Demurtas, D., Finka, A., Hubbell, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+method+for+the+encapsulation+of+biomolecules+into+polymersomes+via+direct+hydration.">A novel method for the encapsulation of biomolecules into polymersomes via direct hydration.</a> Langmuir. 25 (16), 9025-9029 (2009).</li><li>Saad, W. S., Prud'homme, R. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Principles+of+nanoparticle+formation+by+flash+nanoprecipitation.">Principles of nanoparticle formation by flash nanoprecipitation.</a> Nano Today. 11 (2), 212-227 (2016).</li><li>Johnson, B. K., Prud'homme, R. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanism+for+rapid+self-assembly+of+block+copolymer+nanoparticles.">Mechanism for rapid self-assembly of block copolymer nanoparticles.</a> Physical Review Letters. 91 (11), 118302 (2003).</li><li>Han, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simple+confined+impingement+jets+mixer+for+flash+nanoprecipitation.">A simple confined impingement jets mixer for flash nanoprecipitation.</a> Journal of Pharmaceutical Sciences. 101 (10), 4018-4023 (2012).</li><li>Allen, S., Osorio, O., Liu, Y. G., Scott, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Facile+assembly+and+loading+of+theranostic+polymersomes+via+multi-impingement+flash+nanoprecipitation.">Facile assembly and loading of theranostic polymersomes via multi-impingement flash nanoprecipitation.</a> Journal of Controlled Release. 262, 91-103 (2017).</li><li>Bobbala, S., Allen, S. D., Scott, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flash+nanoprecipitation+permits+versatile+assembly+and+loading+of+polymeric+bicontinuous+cubic+nanospheres.">Flash nanoprecipitation permits versatile assembly and loading of polymeric bicontinuous cubic nanospheres.</a> Nanoscale. 10 (11), 5078-5088 (2018).</li><li>Allen, S. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polymersomes+scalably+fabricated+via+flash+nanoprecipitation+are+non-toxic+in+non-human+primates+and+associate+with+leukocytes+in+the+spleen+and+kidney+following+intravenous+administration.">Polymersomes scalably fabricated via flash nanoprecipitation are non-toxic in non-human primates and associate with leukocytes in the spleen and kidney following intravenous administration.</a> Nano Research. , (2018).</li><li>Karabin, N. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sustained+micellar+delivery+via+inducible+transitions+in+nanostructure+morphology.">Sustained micellar delivery via inducible transitions in nanostructure morphology.</a> Nature Communications. 9 (1), 624 (2018).</li><li>Mascoli, C. C., Weary, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Limulus+amebocyte+lysate+(LAL)+test+for+detecting+pyrogens+in+parenteral+injectable+products+and+medical+devices:+advantages+to+manufacturers+and+regulatory+officials.">Limulus amebocyte lysate (LAL) test for detecting pyrogens in parenteral injectable products and medical devices: advantages to manufacturers and regulatory officials.</a> Journal of the Parenteral Drug Association. 33 (2), 81-95 (1979).</li><li>Pustulka, K. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flash+nanoprecipitation:+particle+structure+and+stability.">Flash nanoprecipitation: particle structure and stability.</a> Molecular Pharmaceutics. 10 (11), 4367-4377 (2013).</li><li>Tang, C., Amin, D., Messersmith, P. B., Anthony, J. E., Prud'homme, R. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polymer+directed+self-assembly+of+pH-responsive+antioxidant+nanoparticles.">Polymer directed self-assembly of pH-responsive antioxidant nanoparticles.</a> Langmuir. 31 (12), 3612-3620 (2015).</li><li>Gindy, M. E., Panagiotopoulos, A. Z., Prud'homme, R. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Composite+block+copolymer+stabilized+nanoparticles:+simultaneous+encapsulation+of+organic+actives+and+inorganic+nanostructures.">Composite block copolymer stabilized nanoparticles: simultaneous encapsulation of organic actives and inorganic nanostructures.</a> Langmuir. 24 (1), 83-90 (2008).</li></ol>

Vi gir detaljerte instruksjoner for rask fabrikasjon av polymersomes med PEG17-bl- PPS35-SH som diblock copolymer. Vesicula polymersomes er primære samlet morfologi samlet på dette forholdet hydrofile pinne og hydrofobe PPS blokk molekylvekt. Når impinged flere ganger, de har en diameter og polydispersity som tilsvarer polymersomes ekstrudert gjennom en 200 nm membran etter å ha blitt dannet via tynnfilm hydration. Denne protokollen dermed eliminerer behovet for ytterligere eks...

Nanocarriers tillate kontrollert levering av små og macromolecular Last, inkludert aktive enheter som, hvis ikke innkapslet, ville være enten svært nedbrytbart og/eller for hydrofobe for administrasjon i vivo. Nanocarrier morphologies regelmessig fabrikkert, tilby polymere blemmer tilsvarende liposomer (også kalt polymersomes) muligheten til å samtidig laste hydrofile og hydrofobe Last1,2. Til tross for deres lovende fordeler er polymersomes fremdeles sjeldne i klinisk bruk skyldes, delvis, flere viktige utfordringer i sin produksjon. For klinisk bruk må polymersome formuleringer gjøres i store, sterile og konsekvent grupper.
En rekke teknikker kan brukes til skjemaet polymersomes fra en diblock kopolymer, som poly(ethylene glycol) -blokk-poly (propylen sulfide) (PEG -bl- PPS), som inkluderer løsemiddel spredning3, tynnfilm rehydrering1 , 4, microfluidics 5,6og direkte hydration7. Løsemiddel spredning innebærer lang incubation ganger i nærvær av organiske løsemidler, som kan denature noen bioaktive payloads, som proteiner. Tynnfilm rehydrering tilbyr ikke kontroll over polydispersity av det dannet polymersomes, ofte krever dyre og tidkrevende ekstrudering teknikker for å oppnå akseptabel monodispersity. Videre er både microfluids og direkte hydrering vanskelig å skalere opp større produksjonsvolumer. Av ulike nanocarrier fabrikasjon metoder tilbyr flash nanoprecipitation (FNP) muligheten til å gjøre store og reproduserbar formuleringer8,9,10. Mens FNP var tidligere reservert for utformingen av solid kjerner nanopartikler, vår lab har nylig utvidet bruk av FNP med konsekvent dannelsen av ulike PEG -bl- PPS nanostructure morphologies11, 12, inkludert polymersomes11 og bicontinuous nanospheres12. Vi fant at FNP var stand til å danne monodisperse formuleringer av polymersomes uten behov for ekstrudering, noe som resulterer i overlegen polydispersity indeksverdier sammenlignet med ikke-ekstruderte polymersomes dannet av tynnfilm rehydrering og løsemiddel dispersjon 11. Bicontinuous nanospheres, med sine store hydrofobe domener, klarte ikke å være dannet av tynnfilm rehydration, til tross for danner under en rekke løsemiddel forhold med FNP12.
Her vil vi gi en detaljert beskrivelse for syntese av PEG -bl- PPS diblock copolymer brukes i polymersome-formasjonen, trange impingement jetfly (CIJ) blandebatteri brukes for FNP, FNP protokollen selv, og gjennomføringen av et automatisert system for å redusere bruker variasjon. Inkludert er informasjon om hvordan sterilize systemet tilstrekkelig for å produsere endotoxin-fri formuleringer for bruk i vivoog representant data om karakterisering av polymersomes dannet av FNP. Med denne informasjonen, vil leserne med interesse i å utnytte polymersomes for i vitro og vivo arbeid kunne dikte egne steril, monodisperse formuleringer. Lesere med erfaring i nanocarrier formuleringer og polymer syntese kompetanse vil kunne raskt teste sine egne polymer systemer bruker FNP som et potensielt alternativ til deres nåværende formulering teknikker. Protokollene beskrevet her kan i tillegg brukes som pedagogisk verktøy for utformingen av nanocarriers i nanoteknologi laboratorium kurs.

<table>
	<tbody>
		<tr>
			<td>CanaKit Raspberry Pi 3 Ultimate Starter Kit - 32 GB Edition</td>
			<td>CanaKit</td>
			<td>UPC 682710991511</td>
			<td></td>
		</tr>
		<tr>
			<td>Linear Bearing Platform (Small) - 8mm Diameter</td>
			<td>Adafruit</td>
			<td>1179</td>
			<td></td>
		</tr>
		<tr>
			<td>Linear Motion 8 mm Shaft, 330 mm Length, Chrome Plated, Case Hardened, Metric</td>
			<td>VXB</td>
			<td>kit11868</td>
			<td></td>
		</tr>
		<tr>
			<td>Linear Rail Shaft Guide/Support - 8 mm Diameter</td>
			<td>Adafruit</td>
			<td>1182</td>
			<td></td>
		</tr>
		<tr>
			<td>Manual-Position Precision Slide 4.5&#34; Stroke, 15 lb load capacity</td>
			<td>McMaster-Carr</td>
			<td>5236A16</td>
			<td></td>
		</tr>
		<tr>
			<td>MTPM-P10-1JK43 Iron Horse DC motor</td>
			<td>Iron Horse</td>
			<td>MTPM-P10-1JK43</td>
			<td></td>
		</tr>
		<tr>
			<td>Official Raspberry Pi Foundation 7&#34; Touchscreen LCD Display</td>
			<td>Raspberry Pi</td>
			<td>B0153R2A9I (ASIN)</td>
			<td></td>
		</tr>
		<tr>
			<td>PicoBorg Reverse - Advanced motor control for Raspberry Pi</td>
			<td>PiBorg</td>
			<td>BURN-0011</td>
			<td></td>
		</tr>
		<tr>
			<td>Pololu Carrier with Sharp GP2Y0D810Z0F Digital Distance Sensor 10cm</td>
			<td>Pololu</td>
			<td>1134</td>
			<td></td>
		</tr>
		<tr>
			<td>Ruland PSR16-5-4-A Set Screw Beam Coupling, Polished Aluminum, Inch, 5/16&#34; Bore A Diameter, 1/4&#34; Bore B Diameter, 1&#34; OD, 1-1/4&#34; Length, 44 lb-in Nominal Torque</td>
			<td>Ruland</td>
			<td>PSR16-5-4-A</td>
			<td></td>
		</tr>
		<tr>
			<td>Polyethylene glycol monomethyl ether</td>
			<td>Sigma Aldrich</td>
			<td>202495</td>
			<td></td>
		</tr>
		<tr>
			<td>Methanesulfonyl chloride</td>
			<td>Sigma Aldrich</td>
			<td>471259</td>
			<td></td>
		</tr>
		<tr>
			<td>Toluene</td>
			<td>Sigma Aldrich</td>
			<td>179418</td>
			<td></td>
		</tr>
		<tr>
			<td>Toluene, Anhydrous</td>
			<td>Sigma Aldrich</td>
			<td>244511</td>
			<td></td>
		</tr>
		<tr>
			<td>Triethylamine</td>
			<td>Sigma Aldrich</td>
			<td>T0886</td>
			<td></td>
		</tr>
		<tr>
			<td>Celite 545 (Diatomaceous Earth)</td>
			<td>Sigma Aldrich</td>
			<td>419931</td>
			<td></td>
		</tr>
		<tr>
			<td>Dichloromethane</td>
			<td>Sigma Aldrich</td>
			<td>320269</td>
			<td></td>
		</tr>
		<tr>
			<td>Diethyl ether</td>
			<td>Sigma Aldrich</td>
			<td>296082</td>
			<td></td>
		</tr>
		<tr>
			<td>N,N-Dimethylformamide, anhydrous</td>
			<td>Sigma Aldrich</td>
			<td>227056</td>
			<td></td>
		</tr>
		<tr>
			<td>Potassium carbonate</td>
			<td>Sigma Aldrich</td>
			<td>791776</td>
			<td></td>
		</tr>
		<tr>
			<td>Thioacetic acid</td>
			<td>Sigma Aldrich</td>
			<td>T30805</td>
			<td></td>
		</tr>
		<tr>
			<td>Tetrahydrofuran</td>
			<td>Sigma Aldrich</td>
			<td>360589</td>
			<td></td>
		</tr>
		<tr>
			<td>Aluminum oxide, neutral, activated, Brockmann I</td>
			<td>Sigma Aldrich</td>
			<td>199974</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium methoxide solution, 0.5 M in methanol</td>
			<td>Sigma Aldrich</td>
			<td>403067</td>
			<td></td>
		</tr>
		<tr>
			<td>Propylene sulfide</td>
			<td>Sigma Aldrich</td>
			<td>P53209</td>
			<td></td>
		</tr>
		<tr>
			<td>Acetic acid</td>
			<td>Sigma Aldrich</td>
			<td>A6283</td>
			<td></td>
		</tr>
		<tr>
			<td>Methanol</td>
			<td>Sigma Aldrich</td>
			<td>320390</td>
			<td></td>
		</tr>
		<tr>
			<td>Sodium hydroxide solution 1.0 N</td>
			<td>Sigma Aldrich</td>
			<td>S2770</td>
			<td></td>
		</tr>
		<tr>
			<td>Endotoxin-free water</td>
			<td>GE Healthcare Life Sciences</td>
			<td>SH30529.01</td>
			<td></td>
		</tr>
		<tr>
			<td>Paper pH strips</td>
			<td>Fisher Scientific</td>
			<td>13-640-508</td>
			<td></td>
		</tr>
		<tr>
			<td>Endotoxin-free Dulbecco&#39;s PBS</td>
			<td>Sigma Aldrich</td>
			<td>TMS-012</td>
			<td></td>
		</tr>
		<tr>
			<td>Borosilicate glass scintillation vials</td>
			<td>Fisher Scientific</td>
			<td>03-337-4</td>
			<td></td>
		</tr>
		<tr>
			<td>1 mL all-plastic syringe</td>
			<td>Thermo Scientific</td>
			<td>S75101</td>
			<td></td>
		</tr>
		<tr>
			<td>Sepharose CL-6B</td>
			<td>Sigma Aldrich</td>
			<td>CL6B200</td>
			<td></td>
		</tr>
		<tr>
			<td>Liquid chromatography column</td>
			<td>Sigma Aldrich</td>
			<td>C4169</td>
			<td></td>
		</tr>
		<tr>
			<td>CIJ mixer, HDPE</td>
			<td>Custom</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>Triton X-100</td>
			<td>Sigma Aldrich</td>
			<td>X100</td>
			<td></td>
		</tr>
		<tr>
			<td>Hydrogen peroxide solution</td>
			<td>Sigma Aldrich</td>
			<td>216763</td>
			<td></td>
		</tr>
		<tr>
			<td>HEK-Blue hTLR4</td>
			<td>InvivoGen</td>
			<td>hkb-htlr4</td>
			<td></td>
		</tr>
		<tr>
			<td>RAW-Blue Cells</td>
			<td>InvivoGen</td>
			<td>raw-sp</td>
			<td></td>
		</tr>
		<tr>
			<td>QUANTI-Blue</td>
			<td>InvivoGen</td>
			<td>rep-qb1</td>
			<td></td>
		</tr>
		<tr>
			<td>PYROGENT Gel Clot LAL Assays</td>
			<td>Lonza</td>
			<td>N183-125</td>
			<td></td>
		</tr>
	</tbody>
</table>

rapid, scalable assembly and loading of bioactive proteins and immunostimulants into diverse synthetic nanocarriers via flash nanoprecipitation

Nanomaterialer presenterer en rekke alternativer for å tilpasse kontrollert levering av enkelt og kombinert molekylær nyttelast for terapeutisk og bildebehandling programmer. Denne økte spesifisitet kan ha klinisk inkludert redusert bivirkninger og lavere doser med sterkere. Videre kan i situ målretting og kontrollert modulering av bestemt celle delsett forbedre i vitro og i vivo undersøkelser av grunnleggende biologisk fenomen og undersøke celle-funksjonen. Dessverre har den nødvendige kompetanse i nanoskala vitenskap, kjemi og ingeniører ofte forbyr laboratorier uten erfaring i disse feltene fra fabrikasjon og tilpasse nanomaterialer som verktøy for sine undersøkelser eller kjøretøy for deres strategier. Her gir vi protokoller for syntese og skalerbar montering av et allsidig giftfri blokk copolymer system mottakelig for lettvinte dannelse og lasting av nanoskala biler for biomedisinsk programmer. Flash nanoprecipitation er presentert som en metode for rask fabrikasjon av ulike nanocarriers fra poly(ethylene glycol) -bl-poly (propylen sulfide) copolymers. Disse protokollene vil tillate laboratorier med et bredt spekter av kompetanse og ressurser til å enkelt og reproduserbar dikte avanserte nanocarrier leveringssystemer for programmer. Design og bygging av et automatisert instrument som sysselsetter en høyhastighets sprøytepumpe å lette flash nanoprecipitation prosessen og for å tillate forbedret kontroll over homogenitet, størrelse, morfologi og lasting av polymersome nanocarriers er beskrevet.

Her har vi presentert en enkel protokoll for utformingen av nanocarriers i stand til å laste hydrofile og hydrofobe Last som er trygge for i vivo musen og ikke-menneskelige primas administrasjon11,13. Vi har også inkludert en detaljert protokoll for syntese av polymer brukes i vår representant resultater, sammen med en beskrivelse til fabrikasjon av et egendefinert instrument for mekanisk-kontrollerte impingement løsn...

Watch this Scientific Journal Video about Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation at JoVE.com

Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation

Nanomaterialer har allsidige mekanismer kontrollert terapeutiske leveringsdatoen for både grunnleggende vitenskap og translasjonsforskning programmer, men deres fabrikasjon ofte krever kompetanse som er tilgjengelig i de fleste biomedisinsk laboratorier. Her presenterer vi protokoller for skalerbare fabrikasjon og terapeutiske lasting av ulike selv montert nanocarriers bruker flash nanoprecipitation.

Rask, skalerbar montering og lasting av bioaktive proteiner og Immunostimulants i ulike syntetisk Nanocarriers Via Flash Nanoprecipitation

Nanomaterials present a wide range of options to customize the controlled delivery of single and combined molecular payloads for therapeutic and imaging ...

Research

JoVE Journal

Bioengineering

Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets

Solid-fase Submonomer syntese av Peptoid Polymers og deres Self-Assembly inn Svært Bestilt Nanosheets

Peptoids are a novel class of biomimetic, non-natural, sequence-specific heteropolymers that resist proteolysis, exhibit potent biological activity, and ...

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

Nanotechnology is a relatively new branch of science that involves harnessing the unique properties of particles that are nanometers in scale ...

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold

Fabrikasjon av et bioaktivt, PCL-baserte &quot;Self-fitting&quot; Shape Memory Polymer Stillas

Tissue engineering has been explored as an alternative strategy for the treatment of critical-sized cranio-maxillofacial (CMF) bone defects. Essential to ...

Fabrication of Mechanically Tunable and Bioactive Metal Scaffolds for Biomedical Applications

Fabrikasjon av mekanisk Tunable og Bioaktive Metal Stillas for Biomedical Applications

Biometal systems have been widely used for biomedical applications, in particular, as load-bearing materials. However, major challenges are high stiffness ...

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

A Novel bioreaktor for High Density Dyrking av Diverse mikrobielle samfunn

A novel reactor design, coined a high density bioreactor (HDBR), is presented for the cultivation and study of high density microbial communities. Past ...

Micropatterning and Assembly of 3D Microvessels

Micropatterning og montering av 3D microvessels

In vitro platforms to study endothelial cells and vascular biology are largely limited to 2D endothelial cell culture, flow chambers with polymer or glass ...

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Fabrikasjon nedbrytbar Thermoresponsive Hydrogels på flere lengde skalaer via reaktive ekstrudering, Microfluidics, selvstendig montering, og Electrospinning

While various smart materials have been explored for a variety of biomedical applications (e.g., drug delivery, tissue engineering, bioimaging, etc.), ...

A Simple and Scalable Fabrication Method for Organic Electronic Devices on Textiles

En enkel og skalerbar Fabrication Metode for Organiske Elektroniske enheter på Tekstil

Today, wearable electronics devices combine a large variety of functional, stretchable, and flexible technologies. However, in many cases, these devices ...

Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers

Forutsi gensletting gjennom spatiotemporal kontroll av siRNA-frigivelse fra fotoresvarende polymere nanocarriers

New materials and methods are needed to better control the binding vs. release of nucleic acids for a wide range of applications that require the precise ...

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications

Mønstre bioaktive proteiner eller peptider på Hydrogel bruker fotokjemi for biologiske programmer

There are many biological stimuli that can influence cell behavior and stem cell differentiation. General cell culture approaches rely on soluble factors ...