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DOI :
10.3791/57796-v
July 3rd, 2018
Chapters
0:04
Title
0:54
TRPV1 Mutagenesis
2:19
Generating the Recombinant Bacmid and Baculovirus for Protein Expression
5:14
eTRPV1 and Single-cysteine Mutant Purification
7:18
eTRPV1 Single-cysteine Mutant Site-directed Spin Labeling
8:12
eTRPV1 Spin-labeled Single Cysteine Mutant Reconstitution
9:30
Results: Characterization of the Minimal Cysteine-less TRPV1 Construct and Single-cysteine Mutants used for Spectroscopic Analysis
11:08
Conclusion
この記事では、分光分析用洗剤可溶化 TRPV1 の生化学的な量を取得する具体的な方法について説明します。結合されたプロトコルは、膜制御環境で哺乳類のイオン チャネルの構造と機能の研究を容易にするために合わせることが生化学的な生物物理学的ツールを提供します。
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