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DOI :
10.3791/57836-v
August 2nd, 2018
Chapters
0:04
Title
0:33
Induction of Hypoxia in HEK293A Cells
1:28
Harvesting Cells Cultured Under Hypoxic Conditions
3:30
Preparing Nuclear Extracts
5:52
Immunoprecipitation of Proteins
7:55
Results: HIF-1α Interacts with ARNT at Hypoxia
8:56
Conclusion
在这里, 我们描述了一个共同免疫沉淀协议, 研究在缺氧条件下内源核蛋白的蛋白质相互作用。该方法适用于低氧转录因子与转录联合调节剂相互作用的证明。
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