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DOI :
10.3791/57836-v
August 2nd, 2018
Chapters
0:04
Title
0:33
Induction of Hypoxia in HEK293A Cells
1:28
Harvesting Cells Cultured Under Hypoxic Conditions
3:30
Preparing Nuclear Extracts
5:52
Immunoprecipitation of Proteins
7:55
Results: HIF-1α Interacts with ARNT at Hypoxia
8:56
Conclusion
ここで低酸素の条件の下で内因性の核蛋白質間蛋白質蛋白質の相互作用を研究する co 免疫沈降プロトコルについて述べる。このメソッドは、転写因子と低酸素で転写共役因子との相互作用のデモンストレーションに適しています。
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