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DOI :
10.3791/57844-v
June 27th, 2018
Chapters
0:04
Title
0:46
Growth and Maintenance of Amoebae
1:57
The Growth of P. aeruginosa
2:16
Preparation of Agar Pad, Preparation of Bacteria-amoeba Sample, and Image Acquisition
4:05
Results: Virulence of P. aeruginosa is Upregulated in Surface-attached Populations
4:38
Conclusion
ここでは、ホストとしてD. キイロタマホコリカビ(アメーバ) を用いた浮遊性や表面に接続された細菌の病原性を測定するためのプロトコルを提案する.1 時間の期間にわたって病原性を測定し、ホストを殺すは、蛍光顕微鏡、画像解析を用いた定量化されます。緑膿菌を使用してこのプロトコルを示す.
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