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DOI :
10.3791/57853-v
August 31st, 2018
Chapters
0:04
Title
0:52
Removal of Paraffin
1:44
Pretreatment of Slides for Preparation of RNA Probe Hybridization
3:03
Hybridization of the RNA Probe
4:07
Visualization of the RNA Probe
6:18
Results: Adult Corals Exposed to Heat Stress Show Spatial Expression of RNA Probes
7:07
Conclusion
このプロトコルの目標はパラフィンに埋め込まれているされていると、スライド ガラス上に断面採取したサンゴ大人上の in situハイブリダイゼーションを行うことです。これはパラフィン包埋組織の RNA アンチセンス プローブの空間表現を視覚化するために使用する質的研究法です。
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