Name: single sensillum recordings for locust palp sensilla basiconica
Uploaded: 2018-06-23T15:00:00
Description: Watch this Scientific Journal Video about Single Sensillum Recordings for Locust Palp Sensilla Basiconica at JoVE.com

Questo lavoro è sostenuto da una sovvenzione dalla Fondazione Nazionale Scienze naturali della Cina (No.31472037). Qualsiasi menzione di marchi o prodotti commerciali in questo articolo è solo scopo di fornire informazioni specifiche e non implica una raccomandazione.

1. preparazione degli strumenti e dell'insetto
<ol><li>
Preparazione delle soluzioni di elettrodi e stimoli tungsteno
	<ol>
<li>Difficoltà un nuovo filo di tungsteno (diametro di 0,125 mm, lunghezza 75 mm) in un micromanipolatore e affilarlo in una soluzione di nitrito (NaNO2) di sodio 10% (p/v) in una siringa a 10 V fornita da un alimentatore per circa 1 min sotto uno stereomicroscopio (ingrandimento 40x).</li>
		<li>Immergere il filo di tungsteno affilata ripetutamente nel NaNO2</sub...

<ol>
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B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Variant+ionotropic+glutamate+receptors+as+chemosensory+receptors+in+Drosophila.">Variant ionotropic glutamate receptors as chemosensory receptors in Drosophila.</a> Cell. 136 (1), 149-162 (2009).</li><li>Vosshall, L. B., Stocker, R. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+architecture+of+smell+and+taste+in+Drosophila.">Molecular architecture of smell and taste in Drosophila.</a> Annual Review of Neuroscience. 30, 505-533 (2007).</li><li>de Bruyne, M., Foster, K., Carlson, J. 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R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cuticular+hydrocarbon+pheromones+for+social+behavior+and+their+coding+in+the+ant+antenna.">Cuticular hydrocarbon pheromones for social behavior and their coding in the ant antenna.</a> Cell Reports. 12 (8), 1261-1271 (2015).</li><li>de Bruyne, M., Clyne, P. J., Carlson, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Odor+coding+in+a+model+olfactory+organ:+the+Drosophila+maxillary+palp.">Odor coding in a model olfactory organ: the Drosophila maxillary palp.</a> Journal of Neuroscience. 19 (11), 4520-4532 (1999).</li><li>Syed, Z., Leal, W. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Maxillary+palps+are+broad+spectrum+odorant+detectors+in+Culex+quinquefasciatus.">Maxillary palps are broad spectrum odorant detectors in Culex quinquefasciatus.</a> Chemical Senses. 32 (8), 727-738 (2007).</li><li>Lu, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Odor+coding+in+the+maxillary+palp+of+the+malaria+vector+mosquito+Anopheles+gambiae.">Odor coding in the maxillary palp of the malaria vector mosquito Anopheles gambiae.</a> Current Biology. 17 (18), 1533-1544 (2007).</li><li>Pellegrino, M., Nakagawa, T., Vosshall, L. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single+sensillum+recordings+in+the+insects+Drosophila+melanogaster+and+Anopheles+gambiae.">Single sensillum recordings in the insects Drosophila melanogaster and Anopheles gambiae.</a> Journal of Visualized Experiments. 36, e1725 (2010).</li><li>Grant, A. J., Wigton, B. E., Aghajanian, J. G., O'Connell, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrophysiological+responses+of+receptor+neurons+in+mosquito+maxillary+palp+sensilla+to+carbon+dioxide.">Electrophysiological responses of receptor neurons in mosquito maxillary palp sensilla to carbon dioxide.</a> Journal of Comparative Physiology A. 177 (4), 389-396 (1995).</li><li>Blaney, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+ultrastructure+of+an+olfactory+sensillum+on+the+maxillary+palps+of+Locusta+migratoria+(L.).">The ultrastructure of an olfactory sensillum on the maxillary palps of Locusta migratoria (L.).</a> Cell and Tissue Research. 184 (3), 397-409 (1977).</li><li>Hassanali, A., Njagi, P. G. N., Bashir, M. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chemical+ecology+of+locusts+and+related+acridids.">Chemical ecology of locusts and related acridids.</a> Annual Review of Entomology. 50, 223-245 (2005).</li><li>Chapman, R. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Contact+chemoreception+in+feeding+by+phytophagous+insects.">Contact chemoreception in feeding by phytophagous insects.</a> Annual Review of Entomology. 48, 455-484 (2003).</li><li>Chapman, R. F., Sword, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+importance+of+palpation+in+food+selection+by+a+polyphagous+grasshopper+(Orthoptera:+Acrididae).">The importance of palpation in food selection by a polyphagous grasshopper (Orthoptera: Acrididae).</a> Journal of Insect Behavior. 6, 79-91 (1993).</li><li>Winstanley, C., Blaney, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chemosensory+mechanisms+of+locusts+in+relation+to+feeding.">Chemosensory mechanisms of locusts in relation to feeding.</a> Entomologia Experimentalis et Applicata. 24, 750-758 (1978).</li><li>Blaney, W., Duckett, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+significance+of+palpation+by+the+maxillary+palps+of+Locusta+migratoria+(L.):+an+electrophysiological+and+behavioural+study.">The significance of palpation by the maxillary palps of Locusta migratoria (L.): an electrophysiological and behavioural study.</a> Journal of Experimental Biology. 63, 701-712 (1975).</li><li>Blaney, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrophysiological+responses+of+the+terminal+sensilla+on+the+maxillary+palps+of+Locusta+migratoria+(L.)+to+some+electrolytes+and+non-electrolytes.">Electrophysiological responses of the terminal sensilla on the maxillary palps of Locusta migratoria (L.) to some electrolytes and non-electrolytes.</a> Journal of Experimental Biology. 60, 275-293 (1974).</li><li>Jin, X., Zhang, S., Zhang, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expression+of+odorant-binding+and+chemosensory+proteins+and+spatial+map+of+chemosensilla+on+labial+palps+of+Locusta+migratoria+(Orthoptera:+Acrididae).">Expression of odorant-binding and chemosensory proteins and spatial map of chemosensilla on labial palps of Locusta migratoria (Orthoptera: Acrididae).</a> Anthropod Structure &amp; Development. 35 (1), 47-56 (2006).</li><li>Zhang, L., Li, H., Zhang, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two+olfactory+pathways+to+detect+aldehydes+on+locust+mouthpart.">Two olfactory pathways to detect aldehydes on locust mouthpart.</a> International Journal of Biological Sciences. 13 (6), 759-771 (2017).</li><li>Dweck, H. K. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Olfactory+channels+associated+with+the+Drosophila+maxillary+palp+mediate+short-+and+long-range+attraction.">Olfactory channels associated with the Drosophila maxillary palp mediate short- and long-range attraction.</a> eLife. 5, e14925 (2016).</li><li>Bohbot, J. D., Sparks, J. T., Dickens, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+maxillary+palp+of+Aedes+aegypti,+a+model+of+multisensory+Integration.">The maxillary palp of Aedes aegypti, a model of multisensory Integration.</a> Insect Biochemistry and Molecular Biology. 48, 29-39 (2014).</li><li>Delventhal, R., Kiely, A., Carlson, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrophysiological+recording+from+Drosophila+labellar+taste+sensilla.">Electrophysiological recording from Drosophila labellar taste sensilla.</a> Journal of Visualized Experiments. 84, e51355 (2014).</li><li>Ng, R., Lin, H. H., Wang, J. W., Su, C. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrophysiological+recording+from+Drosophila+trichoid+sensilla+in+response+to+odorants+of+low+volatility.">Electrophysiological recording from Drosophila trichoid sensilla in response to odorants of low volatility.</a> Journal of Visualized Experiments. 125, e56147 (2017).</li><li>Syed, Z., Leal, W. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrophysiological+measurements+from+a+moth+olfactory+system.">Electrophysiological measurements from a moth olfactory system.</a> Journal of Visualized Experiments. 49, e2489 (2011).</li><li>Saha, D., Leong, K., Katta, N., Raman, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multi-unit+recording+methods+to+characterize+neural+activity+in+the+locust+(Schistocerca+Americana)+olfactory+circuits.">Multi-unit recording methods to characterize neural activity in the locust (Schistocerca Americana) olfactory circuits.</a> Journal of Visualized Experiments. 71, e50139 (2013).</li><li>Liu, F., Liu, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+single+sensillum+recording+to+detect+olfactory+neuron+responses+of+bed+bugs+to+semiochemicals.">Using single sensillum recording to detect olfactory neuron responses of bed bugs to semiochemicals.</a> Journal of Visualized Experiments. 107, e53337 (2016).</li></ol>

Gli insetti si basano su palpi per rilevare gli odori degli alimenti, e loro palpi sono creduti per svolgere un ruolo importante nella speciazione13,27. I palpi sono semplici organi olfattivi e stanno ricevendo crescente attenzione come un modello interessante per l'esplorazione della neuromolecular reti sottostanti chemosensation28.
Insetto labellar e palpo SSRs sono stati effettuati con successo su Drosoph...

Gli animali si sono evoluti una gamma di chemosensory organi che percepiscono segnali chimici esogeni. In insetti, organi chemosensory più importanti sono le antenne e i palpi. Su questi organi, diversi tipi di peli chemosensory, chiamati sensilli chemosensory, sono innervati dai neuroni chemosensory (CSNs) entro i peli. CSNs in sensilli chemosensory riconoscere specifici segnali chimici attraverso la trasduzione del segnale da stimoli chimici a potenziali elettrici che vengono successivamente trasferiti fino al sistema nervoso centrale1,2,3 .
CSNs esprimere vari ricevitori chemosensory [ad es., odorant recettori (ORs)], recettori ionotropici recettori (IRs) e recettori gustativi (GRs) sulle loro membrane, che codificano spunti chimici esogeni associati alle varie forme di chemosensation 4,5,6. La caratterizzazione di CSNs è la chiave per la delucidazione dei meccanismi cellulari e molecolari dell'insetto chemorecezione. Ora sensillum singola registrazione (SSR) è una tecnica ampiamente utilizzata per la caratterizzazione di insetto CSNs in sensilli antennali di molti insetti, tra cui mosche7, falene8, coleotteri9, afidi10, locuste11, e le formiche12. Tuttavia, pochi studi hanno applicato un SSR a insetto palpi13,14,15,16,17, perché le particolari strutture di loro sensilli fanno un registrazione elettrofisiologica difficile18.
Sciami di locuste (Orthoptera) è spesso causano di danni alle colture gravi e perdita economica19. I palpi sono creduti per svolgere un ruolo importante nella selezione cibo delle locuste20,21,22,23,24. Due tipi di sensilli chemosensory sono studiati da un microscopio elettronico a scansione (SEM). Di solito, 350 sensilli chaetica e 7-8 sensilli basiconica sono osservati su ogni cupola della locusta palpi18. Chaetica di sensilli sono sensilli gustativi che percepiscono segnali chimici non volatile, mentre sensilli basiconica avere una funzione olfattiva, rilevamento segnali chimici volatili.
Il palpi locusta, i diametri dei socket capelli i sensilli basiconica (ca. 12 µm), sono molto maggiori di quelli di sensilli chaetica (ca. 8 µm)18,25. La parete cuticolare del basiconica di sensilli sui palpi è molto più spessa di quella di sensilli antennali18. Inoltre, la cupola del palpo ha contenuto fluido all'interno di una cuticola altamente flessibile. Queste caratteristiche fanno sì che una penetrazione con un microelettrodo e un'acquisizione di segnali elettrofisiologici buoni è più difficile per sensilli antennali. In questa carta, un protocollo SSR dettagliato ed altamente efficace per locusta palpo sensilli basiconica è presentato con un video.

<table border="0" cellpadding="0" cellspacing="0" style="width:1145px;" width="1145">
	<tbody>
		<tr height="40">
			<td height="40" style="height:40px;width:303px;">Tungsten wire</td>
			<td style="width:140px;">ADVENT</td>
			<td style="width:217px;">W559504</td>
			<td style="width:485px;">Used for making the electrode and fixing the palp</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">NaNO2</td>
			<td>Sigma-aldrich</td>
			<td>563218-25G</td>
			<td>Used for sharpening the tungsten wire</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">AC Power Supply</td>
			<td>Syntech</td>
			<td>A2-70</td>
			<td>Providing the voltage in sharpening the tungsten wire</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Stereoscope</td>
			<td>Motic</td>
			<td>SMZ-163</td>
			<td>Used for observing the sharpening of tungsten wire</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Microscope</td>
			<td>Olympus</td>
			<td>W-51</td>
			<td>Used for observing the sensilla on locust maxillary palp</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Intelligent Data Acquisition Controller</td>
			<td>Syntech</td>
			<td>IDAC-4</td>
			<td>Real-time on screen display of all signals before and during recording</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Stimulus controller</td>
			<td>Syntech</td>
			<td>CS-55</td>
			<td>Used for controlling the stimulus application</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Electronic micromanipulator</td>
			<td>C.M.D.T</td>
			<td>CFT-8301D</td>
			<td>Used for minor movement of the recording electrode</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Micromanipulator</td>
			<td>Narishige</td>
			<td>MN-151</td>
			<td>Used for minor movement of the reference electrode</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Speaker</td>
			<td>EDIFIER</td>
			<td>R101T06</td>
			<td>Connected with IDAC-4 and providing sound for the signal</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Magnetic base</td>
			<td>PDOK</td>
			<td>PD-101</td>
			<td>Used to hold the electrode, and stimulus delivery tube</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Vibration Isolation Table</td>
			<td>TianHe</td>
			<td>HAP-100-1208</td>
			<td>Used for isolating the vibration from the equipment</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Glass slide</td>
			<td>CITOGLAS</td>
			<td>ZBP-407</td>
			<td>Used for making the base for the MPH</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Blu-tack</td>
			<td>Bostik</td>
			<td>Blu-tack-45g</td>
			<td>Fixing the tungsten wire</td>
		</tr>
		<tr height="32">
			<td height="32" style="height:32px;">Pasteur tube</td>
			<td>YARE</td>
			<td>WITEG</td>
			<td>Placing the filter paper containing stimuli solutions</td>
		</tr>
	</tbody>
</table>

single sensillum recordings for locust palp sensilla basiconica

I palpi dell'apparato boccale locusta sono considerati organi gustativi convenzionali che svolgono un ruolo importante nella selezione di cibo di una locusta, soprattutto per il rilevamento dei segnali chimici non volatile attraverso sensilli chaetica (precedentemente denominato terminal sensilli o crestati sensilli). C'è ora aumentando la prova che questi palpi hanno anche una funzione olfattiva. Un recettore odorizzante (LmigOR2) e un odorant-binding protein (LmigOBP1) sono state localizzate nei neuroni e nelle cellule accessorio, rispettivamente, la basiconica di sensilli dei palpi. Single sensillum (SSR) di registrazione utilizzato per registrare le risposte dei neuroni odorant, che è un metodo efficace per lo screening di ligandi attivi sui recettori specifici odoranti. SSR è utilizzata negli studi funzionali dei recettori odorant in sensilli palpo. La struttura del basiconica di sensilli situato sulla cupola dei palpi differisce da piuttosto dalla struttura di quelli sulla antenne. Di conseguenza, quando si esegue un SSR suscitato da odoranti, qualche consiglio specifico può essere utile per ottenere risultati ottimali. In questa carta, è stato introdotto un protocollo dettagliato e altamente efficace per un relè SSR da insetto palpo sensilli basiconica.

Due sottotipi di sensilli (pb1 e pb2) sul palpo mascellare locuste vengono identificati in base a dinamiche di risposta diversa per chimica odoranti (isoottanolo 1 10% e 10% acido nonanoico). I neuroni in pb1 producono picchi significativamente più di 1-isoottanolo rispetto ad acido nonanoico mentre i neuroni in pb2 sono significativamente che meno attivata da 1 isoottanolo rispetto ad acido nonanoico (Figura 4). Esanale ed E-2-esenale può evocare un palpo ...

Watch this Scientific Journal Video about Single Sensillum Recordings for Locust Palp Sensilla Basiconica at JoVE.com

Single Sensillum Recordings for Locust Palp Sensilla Basiconica

Questo articolo descrive un protocollo dettagliato ed altamente efficace per le registrazioni di single sensillum dalla basiconica di sensilli sul palpi dell'apparato boccale degli insetti.

Sensillum singole registrazioni per Locust Palp sensilli Basiconica

The palps of locust mouthparts are considered to be conventional gustatory organs that play an important role in a locust&#39;s food selection, especially ...

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Neuroscience

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae

Registrazioni Sensillum singolo in Insetti Drosophila melanogaster E Anopheles gambiae</em

The sense of smell is essential for insects to find foods, mates, predators, and oviposition sites3. Insect olfactory sensory neurons (OSNs) are enclosed ...

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Neuroscienze

Single-unit In vivo Recordings from the Optic Chiasm of Rat

Single-unit In vivo Recordings from the Optic Chiasm of Rat

Singola unità In vivo Registrazioni dal chiasma ottico di Rat

Information about the visual world is transmitted to the brain in sequences of action potentials in retinal ganglion cell axons that make up the optic ...

Electrophysiology of Scorpion Peg Sensilla

Elettrofisiologia di Scorpion Peg sensilli

We describe a modification of an existing tip-recording technique1,2 for electrophysiologically investigating short, peg-like sensory sensilla3,4. On the ...

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits

Multi-unit Recording Methods to Characterize Neural Activity in the Locust Schistocerca Americana Olfactory Circuits

Multi-unit Metodi di registrazione per caratterizzare l&#39;attività neurale nel Locust (<em&gt; Schistocerca Americana</em&gt;) Circuiti olfattive

Detection and interpretation of olfactory cues are critical for the survival  of many organisms. Remarkably, species across phyla have strikingly similar ...

A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals

Una procedura per l&#39;impianto Array organizzata di microfili per Recordings singola unità di Awake, Comportarsi Animali

In vivo electrophysiological recordings in the awake, behaving animal provide a powerful method for understanding neural signaling at the single-cell ...

Electrophysiological Recording From Drosophila Labellar Taste Sensilla

Electrophysiological Recording From Drosophila Labellar Taste Sensilla

Elettrofisiologici Registrazione da<em&gt; Drosophila</em&gt; Labellar Taste Sensilla

The peripheral taste response of insects can be powerfully investigated with electrophysiological techniques. The method described here allows the ...

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Associati a cellule intere registrazioni in organotipiche fettine di ippocampo

Pair recordings involve simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling not only direct ...

Using Single Sensillum Recording to Detect Olfactory Neuron Responses of Bed Bugs to Semiochemicals

Utilizzando Sensillum singolo controllo per rilevare olfattiva Neuron Risposte di cimici a semiochimici

The insect olfactory system plays an important role in detecting semiochemicals in the environment. In particular, the antennal sensilla which house ...

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization

Localizzazione dei geni del recettore degli odoranti nell&#39;antenna di Locust da RNA<em&gt; In Situ</em&gt; Ibridazione

Insects have evolved sophisticated olfactory reception systems to sense exogenous chemical signals. These chemical signals are transduced by Olfactory ...

Electrophysiological Recording from Drosophila Trichoid Sensilla in Response to Odorants of Low Volatility

Electrophysiological Recording from Drosophila Trichoid Sensilla in Response to Odorants of Low Volatility

Registrazione elettrofisiologica da<em&gt; Drosophila</em&gt; Senso Trichoide in risposta a Odoranti di bassa volatilità

Insects rely on their sense of smell to guide a wide range of behaviors that are critical for their survival, such as food-seeking, predator avoidance, ...