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DOI :
10.3791/57872-v
August 17th, 2018
Chapters
0:04
Title
0:39
Preparation of Primary Cells from Zebrafish Embryos
1:54
Preparation of Reusable Poly-L-lysine-coated Glass Bottom Dishes
3:26
Preparation and Plating of Primary Cells
6:02
Transfection of Primary Cells with Plasmid DNA
6:57
Results: Live Cell Imaging of Zebrafish Primary Cells
8:25
Conclusion
我们提出了一个有效的和易于使用的协议, 以制备斑马鱼胚胎的原细胞培养的转染和活细胞成像, 以及一个协议, 以准备初级细胞从成年斑马鱼的大脑。
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