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DOI :
10.3791/57884-v
•
9:16 min
June 26th, 2018
Chapters
0:04
Title
0:36
Isolation of Microsomes-associated Alpha-synuclein Aggregates from Diseased A53T Alpha-synuclein Transgenic Mic
2:30
Western Blot
4:32
Neurons Treatment
5:40
Immunofluorescence
7:45
Results: Time-dependent Induction of Alpha-synuclein Deposition After Administration of Microsomes-associated Alpha-synuclein Aggregates
8:35
Conclusion
このプロトコルの目的は、生体内でα-シヌクレインの形成を複製するセルベースのシステムを集約を提供することです。細胞内の α-シヌクレイン介在物は一次ニューロンに内面化することによって播かれ、伝搬の外因性投与罹患 α-シヌクレイン遺伝子組換えマウスから隔離されたネイティブのミクロソームによる α-シヌクレイン凝集体。
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