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DOI :
10.3791/57902-v
•
9:30 min
August 6th, 2018
Chapters
0:04
Title
1:28
Assembly of Prepore Anthrax Toxin Complex on a PDEA-modified Amine Reactive Biosensor Surface
4:37
Assembly of Pore Anthrax Toxin Complex on PDEA-modified Amine Reactive Biosensor Surface
5:26
Identification of Released Macromolecular Assemblies from BLI Biosensors by Negative Stain Electron Microscopy and Mass Spectrometry
6:50
Results: Analysis of Dynamic Protein Complexes by Biolayer Interferometry, Electron Microscopy, and Mass Spectrometry
8:29
Conclusion
在这里, 我们提出了一个协议, 以监测炭疽毒素的组装和拆卸使用 biolayer 干涉测量 (BLI)。在生物传感器表面组装/拆卸后, 通过电子显微镜和质谱法, 从表面释放出大量的蛋白质复合物, 用于可视化和识别复合物的成分。
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