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DOI :
10.3791/57921-v
June 10th, 2018
Chapters
0:04
Title
1:14
Bulk Stabilization of Conventional hPSC Cultures for Subsequent Naïve Reversion with a Brief LIF-5i Adaptation
4:38
Long-term Maintenance and Expansion of N-hPSC in LIF-3i Medium
7:14
Results: Effective Reversion of Human Pluripotent Stem Cells
7:47
Conclusion
効率的な一括、および急速な化学復帰従来系列プライミングひと多能性幹細胞 (hPSC) の epigenomically 安定した素朴な着床前胚盤葉上層のような多能性状態にするためのプロトコルを提案します。このメソッド結果減少系統プライミング遺伝子発現と分化多能でマーク付きの改善で従来の hPSC ラインの幅広いレパートリー。
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