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DOI :
10.3791/57971-v
July 27th, 2018
Chapters
0:04
Title
0:40
Cell Seeding and Treatment
2:46
Cell Harvest and Preparation for Electrodisruption
3:53
Plasma Membrane Electrodisruption and Separation of Sedimentable- and Total Cell Fractions
5:50
LDH Extraction and Measurement of LDH Enzymatic Activity
6:55
Results: Monitoring Bulk Autophagic Sequestration Activity in Mammalian Cells
8:43
Conclusion
本文介绍了一种简便、有效的测量哺乳动物细胞中大块自噬性螯合活性的协议。该方法以定量测定乳酸脱氢酶 (ldh) 在 sedimentable 细胞分数与总细胞 LDH 水平的比例。
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