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DOI :
10.3791/57971-v
July 27th, 2018
Chapters
0:04
Title
0:40
Cell Seeding and Treatment
2:46
Cell Harvest and Preparation for Electrodisruption
3:53
Plasma Membrane Electrodisruption and Separation of Sedimentable- and Total Cell Fractions
5:50
LDH Extraction and Measurement of LDH Enzymatic Activity
6:55
Results: Monitoring Bulk Autophagic Sequestration Activity in Mammalian Cells
8:43
Conclusion
ここで哺乳類細胞における一括 autophagic 貯留活性を測定する簡単なよく検証されたプロトコルが記述されています。メソッドは、sedimentable セル画総細胞 LDH レベルと比較して乳酸脱水素酵素 (LDH) の割合を定量化に基づいています。
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