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DOI :
10.3791/57998-v
•
6:48 min
October 2nd, 2018
Chapters
0:04
Title
0:25
Preparation of the Primers and Expression Vectors
2:05
Ligating the Annealed gRNA Oligonucleotides
2:59
Transformation of E. coli
4:03
PCR Analysis
5:35
Results: CRISPR Guided RNA Cloning
6:23
Conclusion
여기, 간단 하 고, 효율적인, 그리고 sgRNA 복제의 비용 효율적인 방법 설명입니다.
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