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DOI :
10.3791/58020-v
July 26th, 2018
Chapters
0:04
Title
0:49
Alkaline Hydrolysis of Genomic DNA
2:36
Strand specific Southern Blot: Transfer
4:37
Strand-specific Southern Blot: Hybridization
6:48
Results: Assessment of Ribonucleotide Incorporation into DNA
8:08
Conclusion
Ribonucleotides 是在核 DNA 复制过程中纳入基因组的最丰富的非规范核苷酸之一。如果没有正确去除, ribonucleotides 会导致 DNA 损伤和突变。在这里, 我们提出了两个实验方法, 用于评估核苷酸的丰度纳入 DNA 及其诱变效应。
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