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DOI :
10.3791/58034-v
August 16th, 2018
Chapters
0:04
Title
0:49
Generation and Maintenance of Astrocyte Progenitors (hAstros) from hPSCs
2:41
Generation and Maintenance of Neurons (hNeurons) and Inducible Neurons (iNeurons) from hPSCs
3:27
Preparation and Maintenance of 3D Sphere Co-cultures
5:42
Measurement of Live Synaptic Physiology with Multielectrode Arrays (MEAs)
6:56
Results: Morphological and Physiological Characteristics of Synaptic Microcircuits
8:07
Conclusion
このプロトコルの結合解離ひと多能性幹細胞由来神経細胞の再現性のある手法を目指して、アストロ サイト 3 D に一緒に球共培養系、これら球無料浮動小数点条件とその後の維持免疫分析によって行い電極の録音と球のシナプス回路活性を測定します。
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