Name: using a knee arthrometer to evaluate tissue-specific contributions to knee flexion contracture in the rat
Uploaded: 2018-11-09T13:00:00
Description: Watch this Scientific Journal Video about Using a Knee Arthrometer to Evaluate Tissue-specific Contributions to Knee Flexion Contracture in the Rat at JoVE.com

作者要感谢 joao tomas 在该设备方面提供的技术援助, 并感谢 khaoula louati 在开发图像分析方法方面提供的协助。

所使用的大鼠膝盖固定模型已获得渥太华大学动物护理和兽医服务机构以及当地伦理委员会的批准。
1. 动物准备
<ol><li>在预定的固定时间结束时, 通过给老鼠排放二氧化碳来安乐死。 注: 在这里, 我们使用了一个固定模型与板和2个螺丝 (一个插入在股骨近端, 另一个在胫骨远端), 避免违反任何膝关节结构, 并保持膝盖弯曲的位置 135°, 如前面所述6.在?...

<ol>
	<li>Clavet, H., H&#233;bert, P. C., Fergusson, D., Doucette, S., Trudel, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Joint+contracture+following+prolonged+stay+in+the+intensive+care+unit.">Joint contracture following prolonged stay in the intensive care unit.</a> Canadian Medical Association Journal. 178 (6), 691-697 (2008).</li><li>Campbell, T. M., Dudek, N., Trudel, G., Silver, J. K., Frontera, W. R., Rizzo, T. 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A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stretch+for+the+treatment+and+prevention+of+contractures.">Stretch for the treatment and prevention of contractures.</a> Cochrane Database of Systematic Reviews. 1, Cd007455 (2017).</li><li>Trudel, G., Himori, K., Uhthoff, H. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Contrasting+alterations+of+apposed+and+unapposed+articular+cartilage+during+joint+contracture+formation.">Contrasting alterations of apposed and unapposed articular cartilage during joint contracture formation.</a> Archives of Physical Medicine Rehabilitation. 86 (1), 90-97 (2005).</li><li>Trudel, G., Uhthoff, H. 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A., Sutherland, C., Zhang, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rabbit+knee+model+of+post-traumatic+joint+contractures:+the+long-term+natural+history+of+motion+loss+and+myofibroblasts.">Rabbit knee model of post-traumatic joint contractures: the long-term natural history of motion loss and myofibroblasts.</a> Journal of Orthopaedic Research . 22 (2), 313-320 (2004).</li><li>Klein, L., Player, J. S., Heiple, K. G., Bahniuk, E., Goldberg, V. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isotopic+evidence+for+resorption+of+soft+tissues+and+bone+in+immobilized+dogs.">Isotopic evidence for resorption of soft tissues and bone in immobilized dogs.</a> Journal of Bone and Joint Surgery. American Volume. 64 (2), 225-230 (1982).</li></ol>

开发了大鼠膝关节关节仪, 可重现、可靠地确定干预后大鼠膝关节的最大伸长率。该装置的优点包括在膝关节上持续产生扭矩, 手臂长度和延长力保持不变。另一个优势包括能够将扭矩设置在允许在同一关节上重复测试的水平, 以评估不同关节结构对膝关节 om 的影响, 如肌肉、胶囊或韧带。例如, 在对完全完整的关节进行测试后, 可以分割后关节膝关节肌肉, 重复使用关节造影仪测试, 以确定关节源?...

关节有全范围的运动 (rom) 对健康和幸福至关重要1。在联合被动 rom 的损失叫收缩 2。关节收缩可能产生于多种情况, 包括长期卧床休息, 瘫痪, 关节置换术, 烧伤, 感染, 和神经疾病1,3,4,5。膝盖收缩可能会致残, 因为它会加速关节退化, 增加跌倒的风险, 并对一个人执行步行、坐着和爬楼梯等基本功能任务的能力产生不利影响.7。
一旦建立, 膝关节收缩是很难治疗, 因此, 确定哪些患者是在发展这种条件的最高风险是必不可少的预防和避免收缩相关的发病率8。实验被设计评估 1) 导致或影响膝关节收缩的情况, 2) 收缩的严重程度, 3) 他们的世俗进展, 4) 参与收缩的组织, (5) 他们的可逆性并且 (6) 有用性对膝关节 rom 的各种预防和治疗干预。对于所有这些实验, 有效的、客观的、精确的和可重复的 rom 测量方法至关重要。其他辅助措施 (能量消耗、组织形态测量、基因表达和蛋白质含量) 是了解关节收缩病理生理的有用标志, 但机械限制是限制患者并导致残疾的原因。在这一研究领域的一些挑战包括异质方法, 通过实验测试膝关节 rom, 以及缺乏定量数据9。使用各种不同的实验方法会产生从实验室到实验室都无法比较的结果。这引起了关于导致关节收缩的条件 (如固定或关节置换术) 的争议.因此, 需要一种在干预后进行实验测量关节 rom 的自动化方法。
在这里, 我们描述了一个用户独立, 有效, 精确和可重复的协议, 用于评估大鼠膝关节 rom 使用定制的关节计连接到数码相机, 以精确测量膝关节 rom 的扩展。我们测试了不同时期的固定对膝关节 rom 的影响。然后, 我们描述了使用固定的骨地标在预先指定的扭矩下测量生成的数字图像上的 rom 的方法。总体而言, 这些方法能可靠地测量大鼠膝关节 rom, 并提供定量数据。

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			<td height="64" style="height:64px;width:251px;">Arthrometer</td>
			<td style="width:205px;">The Ottawa Hospital Rehabilitation Centre - Rehabilitation Engineering&#160;</td>
			<td style="width:119px;">N/A</td>
			<td style="width:167px;"></td>
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			<td height="43" style="height:43px;width:251px;">Camera</td>
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			<td style="width:119px;">EOS-500D</td>
			<td style="width:167px;">Commonly known as EOS Rebel T1i</td>
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			<td height="22" style="height:22px;width:251px;">ImageJ</td>
			<td style="width:205px;">National Institutes of Health</td>
			<td style="width:119px;">Version 1.45s</td>
			<td style="width:167px;"></td>
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			<td style="width:167px;">Kit Includes:&#160; Forceps: medium points, nickel-plated 
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			<td style="width:205px;">Fine Science Tools</td>
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			<td style="width:205px;">Stanley</td>
			<td style="width:119px;">057-3558-2</td>
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			<td height="18" style="height:18px;">Universal AC to DC powder adapter</td>
			<td>RCA</td>
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using a knee arthrometer to evaluate tissue-specific contributions to knee flexion contracture in the rat

正常的膝盖运动范围 (rom) 对健康至关重要, 并允许一个人进行基本的活动, 如步行, 爬楼梯和坐。丢失的 rom 被称为关节收缩, 导致发病率上升。由于很难扭转已建立的膝关节收缩, 早期发现是重要的, 因此, 知道他们的发展的危险因素是必不可少的。由于大鼠膝关节解剖与人膝关节解剖相似, 大鼠能够承受弯曲时膝关节固定的长时间, 以及机械数据可以, 大鼠可以用这种模型研究干预的效果。与膝关节组织的组织学和生化分析有关。
我们使用自动关节仪, 演示了一种经过验证的、精确的、可重复的、与用户无关的方法来测量大鼠膝关节在特定扭矩下的扩展 rom。该关节仪可用于确定干预对大鼠膝关节 rom 的影响。

总结了不同不动期确定的膝关节伸长量, 以增加不动的持续时间, 并表明在固定时间增加后, 会产生更严重的收缩。使用 imagej 的代表性结果如图 3所示。
能够以有效、精确和可重复的、独立于用户的方式测量大鼠膝盖的最大延伸, 从而减少数据中的偏差。在所提供的例子中, 我们评估了7只大鼠在固定4周后?...

Watch this Scientific Journal Video about Using a Knee Arthrometer to Evaluate Tissue-specific Contributions to Knee Flexion Contracture in the Rat at JoVE.com

Using a Knee Arthrometer to Evaluate Tissue-specific Contributions to Knee Flexion Contracture in the Rat

用膝关节关节仪评价大鼠膝关节屈曲收缩的特定组织贡献

该方案的目的是测量大鼠膝关节运动的扩展范围。各种疾病增加膝关节僵硬的影响和治疗的有效性可以量化。

Normal knee range of motion (ROM) is critical to well-being and allows one to perform basic activities such as walking, climbing stairs and sitting. Lost ...

此方法可以帮助回答肌肉骨骼领域的关键问题，例如使用大鼠模型干预对膝关节运动范围的影响。该技术的主要优点是，它提供了一种精确、可重复、用户独立的测量鼠膝关节延伸的方法。首先使用剪刀和钳子去污的安乐死大鼠的下肢，并去除皮肤从底层筋膜。然后，将身体放在其一侧，实验腿朝上。将股骨放在集成在关节计安装阶段的凹槽金属夹中。用精密螺丝刀通过肌肉打孔，将夹子端放在更大的肌管上，以固定股骨。然后，在关节计旋转中心上调整横向股骨。将可移动臂放在腿部后面两个直立柱的位置，在电机激活后，将膝盖推入被动延伸。使用六角钥匙拧紧底座夹紧，直到固定。确保使用螺丝刀将相机正确安装在测量仪上。检查相机是否位于手动对焦上，然后将相机对焦在女性对焦上。根据所测试的膝关节运动范围方向和大鼠的位置，顺时针或逆时针设置关节计的方向。通过同时按下电源和启动按钮激活关节计电机。关节计电机将以 6.6 rpm 的速度移动，然后在达到第一个预设扭矩时停止 2.1 秒。当达到第一个扭矩时，相应的 LED 指示灯将点亮，数码相机将自动拍摄膝盖的照片。拍摄照片后，关节计将继续到下一个更高的预设扭矩。一旦应用了四个扭矩，关节计将停止。一旦大鼠定位在关节仪上并启动测试，测试单膝的总时间约为 18.8 秒。要分离收缩的关节原生或非肌肉成分，请重置扭矩序列以释放施加在腿部的力，然后对后侧关节肌肉进行肌切除术。解剖肌肉近向膝盖关节，以确保胶囊不切割。在肌切除术前和之后测试两条腿，并解剖出任何所需的组织后，按照机构协议处理动物尸体和所有生物危害物质，并清洁关节计。打开包含安装在图像 J 中关节仪上的相机拍摄的数字图像的文件。从主工具栏中选择角度工具，通过绘制股骨夹中间到侧侧凹面的股骨线和从侧股股骨到侧毛的股骨线到侧侧毛角，跟踪股骨-骨角。使用测量工具，单击"分析"然后"测量"以显示上面绘制的两条线产生的计算角度。使用零度约定表示完全扩展。这图显示，与非固定反向肢体相比，7只大鼠在16周固定后最大膝盖延长。对于固定膝盖，最大延长能力比反面的降低。在肌切除术之后，实验膝和反向膝的最大延长能力增强，然而，实验膝盖继续表现出弯曲收缩。在尝试此程序时，确保股骨夹安全良好，以避免在测试期间膝盖运动，这一点很重要。按照这个程序，其他方法，如组织分析膝盖，关节结构可以回答与组织和细胞变化有关的其他问题，发生在膝盖关节，以影响其运动范围。

Research

JoVE Journal

Medicine

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一种生成周期负荷诱导的大鼠膝关节内软骨病变模型的非侵入性方法

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科研

医学

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成人膝关节穿刺术

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Neuropathic pain is a prevalent condition that affects 6.9%-10% of the population and results from nerve damage due to various etiologies, such as lumbar ...

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推拿干预膝骨关节炎兔模型

Knee osteoarthritis (KOA) is mainly characterized by degenerative changes in the knee joint's cartilage and surrounding soft tissues. The efficacy of ...

Experimental Modeling of Knee Osteoarthritis in Rats

Experimental Modeling of Knee Osteoarthritis in Rats (Video) | JoVE 

大鼠膝关节骨关节炎的实验建模

Establishment of the KOA Model in Rabbits Using the Modified Videman Method

Application of Acupotomy in a Knee Osteoarthritis Model in Rabbit

Author Spotlight: Investigating the Mechanism of Action of Acupotomy in Treating Knee Osteoarthritis

Knee osteoarthritis (KOA) is one of the most frequently encountered diseases in the orthopedic department, which seriously reduces the quality of life of ...

Therapeutic Potential of Tuina Massage for Knee Osteoarthritis

Tuina Intervention in Sodium Monoiodoacetate Injection-Induced Rat Model of Knee Osteoarthritis

Treating Knee Osteoarthritis with Tuina - A New Perspective (Video) | JoVE

单碘乙酸钠注射液诱导膝骨关节炎大鼠模型的推拿干预

Knee osteoarthritis (KOA), a common degenerative joint disorder, is characterized by chronic pain and disability, which can progress to irreparable ...

Training Prior to Executing Tuina on Knee Osteoarthritis Rabbit Model

膝骨关节炎兔模型推拿前训练