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DOI :
10.3791/58097-v
•
10:57 min
August 14th, 2018
Chapters
0:04
Title
0:41
Tissue Preparation
1:30
Equipment and Reagent Preparation
3:24
Sample Deparaffinization, Dehydration, and Pre-treatment
5:26
Probe Hybridization, Signal Amplification, and Detection
7:18
Counterstaining, Slide Mounting, and Visualization
8:18
Results: Detection of Splice Variants, Short Sequences, and Point Mutation In Situ
10:00
Conclusion
在这里, 我们描述了原位杂交检测, 使敏感和特定的检测序列短于50核苷酸的单核苷酸分辨率在单细胞水平。该检测可以手动或自动执行, 可以在组织上下文中实现拼接变体、短序列和突变的可视化。
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