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DOI :
10.3791/58118-v
August 22nd, 2018
Chapters
0:04
Title
0:46
Mouse Bone Marrow (BM) and Secondary Lymphoid Tissue Cell Isolation
3:40
In Vivo Activation, Proliferation, and Th1 Cell Differentiation Assay
6:48
Results: Representative BM-derived Dentritic Cell-induced Naïve CD4+ T Cell Activation, Proliferation, and Th1 Differentiation
7:39
Conclusion
在这里, 我们提出了一种在体内测定由 GM-脑脊液骨髓 (BM) 衍生树突状细胞 (CD4) 诱导的天真的 t 细胞 (t 细胞) 活化、增殖和 Th1 分化的方法。此外, 该协议还描述了 BM 和 t 细胞分离、dc 生成和 dc 和 t 细胞的移植。
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