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DOI :
10.3791/58162-v
•
10:14 min
November 1st, 2018
Chapters
0:04
Title
1:05
Isolation of Rat Kidneys
2:17
Kidney Tissue Mincing
3:18
Kidney Tissue Sieving
7:12
Results: Assessment of Efficient Isolation and Cell Viability of Isolated Glomeruli
8:59
Conclusion
このプロトコルの主な焦点は、効率的にさまざまな下流のアプリケーションで使用するための最小限の汚染物質と実行可能な主な糸球体の文化を分離することです。孤立した糸球体コンポーネント セル型の構造の関係を維持、培養前のヴィヴォ短時間のためにすることができます。
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