Name: noninvasive eeg recordings from freely moving piglets
Uploaded: 2018-07-13T15:00:00
Description: Watch this Scientific Journal Video about Noninvasive EEG Recordings from Freely Moving Piglets at JoVE.com

我们要感谢 Scheu 的机会, 在猪圈 Hofgut Neumühle 进行我们的研究。

所有程序均经当地道德委员会批准 (#23177 07/G10-1-010/G 15-15-011), 并遵循欧洲和德国国家条例 (欧洲共同体理事会指令, 86/609/ECC;Tierschutzgesetz)。
所有动物程序都是按照古腾堡的医疗中心-大学美因法动物保育委员会的规定进行的。
1. 设置
<ol><li>在实验之前, 检查任何线路噪音, 并找到一个适当的位置, 设置和天线。线噪声是可见的60或50赫兹正弦波。 注意: 天...

<ol>
	<li>Conrad, M. S., Sutton, B. P., Dilger, R. N., Johnson, R. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+in+vivo+three-dimensional+magnetic+resonance+imaging-based+averaged+brain+collection+of+the+neonatal+piglet+(Sus+scrofa).">An in vivo three-dimensional magnetic resonance imaging-based averaged brain collection of the neonatal piglet (Sus scrofa).</a> PLoS ONE. 9 (9), e107650 (2014).</li><li>de Camp, N. V., Hense, F., Lecher, B., Scheu, H., Bergeler, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Models+for+preterm+cortical+development+using+non+invasive+clinical+EEG.">Models for preterm cortical development using non invasive clinical EEG.</a> Translational Neuroscience. 8, 211-224 (2017).</li><li>Lapray, D., Bergeler, J., Dupont, E., Thews, O., Luhmann, H. J., Barculo, D., Daniels, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+telemetric+system+for+recording+brain+activity+in+small+animals.">A novel telemetric system for recording brain activity in small animals.</a> Telemetry: Research, Technology and Applications. , 195-203 (2009).</li><li>Kim, D., Yeon, C., Kim, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+and+experimental+validation+of+a+dry+non-+invasive+multi-channel+mouse+scalp+EEG+sensor+through+visual+evoked+potential+recordings.">Development and experimental validation of a dry non- invasive multi-channel mouse scalp EEG sensor through visual evoked potential recordings.</a> Sensors. 17, 326 (2017).</li><li>Moshayedi, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+relationship+between+glial+cell+mechanosensitivity+and+foreign+body+reactions+in+the+central+nervous+system.">The relationship between glial cell mechanosensitivity and foreign body reactions in the central nervous system.</a> Biomaterials. 35, 3919-3925 (2014).</li><li>Barrese, J. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Failure+mode+analysis+of+silicon-based+intracortical+microelectrode+arrays+in+non-human+primates.">Failure mode analysis of silicon-based intracortical microelectrode arrays in non-human primates.</a> Journal of Neural Engineering. 10, 066014 (2013).</li><li>Hellstr&#246;m-Westas, L., Ros&#233;n, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electroencephalography+and+brain+damage+in+preterm+infants.">Electroencephalography and brain damage in preterm infants.</a> Early Human Development. 81, 255-261 (2005).</li><li>Lloyd, R. O., Goulding, R. M., Filan, P. M., Boylan, G. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Overcoming+the+practical+challenges+of+electroencephalography+for+very+preterm+infants+in+the+neonatal+intensive+care+unit.">Overcoming the practical challenges of electroencephalography for very preterm infants in the neonatal intensive care unit.</a> Acta Paediatrica. , 152-157 (2015).</li><li>Clancy, B., Finlay, B. L., Darlington, R. B., Anand, K. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Extrapolating+brain+development+from+experimental+species+to+humans.">Extrapolating brain development from experimental species to humans.</a> Neurotoxicology. 28, 931-937 (2007).</li><li>Iyer, K. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cortical+burst+dynamics+predict+clinical+outcome+early+in+extremely+preterm+infants.">Cortical burst dynamics predict clinical outcome early in extremely preterm infants.</a> Brain. 138, 2206-2218 (2015).</li><li>Luhmann, H., de Camp, N., Bergeler, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monitoring+brain+activity+in+preterms:+mathematics+helps+to+predict+clinical+outcome.">Monitoring brain activity in preterms: mathematics helps to predict clinical outcome.</a> Brain. 138, 2114-2125 (2015).</li><li>Dragomir, A., Akay, Y., Curran, A. K., Akay, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Complexity+measures+of+the+central+respiratory+networks+during+wakefulness+and+sleep.">Complexity measures of the central respiratory networks during wakefulness and sleep.</a> Journal of Neural Engineering. 5, 254-261 (2008).</li><li>Peever, J., Fuller, P. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+biology+of+REM+sleep.">The biology of REM sleep.</a> Current Biology. 27, R1237-R1248 (2017).</li><li>Robert, S., Dallaire, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polygraphic+Analysis+of+the+sleep-wake+states+and+the+REM+Sleep+periodicity+in+domesticated+pigs+(Sus+scrofa).">Polygraphic Analysis of the sleep-wake states and the REM Sleep periodicity in domesticated pigs (Sus scrofa).</a> Physiology &amp; Behavior. 37 (2), 289-293 (1986).</li></ol>

该协议的一个关键步骤是适当的皮肤接触与电极, 特别是接地电极, 以实现稳定的录音, 低噪音。此外, 由于仔猪非常敏捷, 所以必须用硅橡胶覆盖整个系统, 以保护电极和遥测装置。此外, 如果实验是在一个稳定的板条地板, 小心与小设备或连接器。
如果不充分掌握自粘性水凝胶电极, 尽量去除磨料奶油尽可能。另一种去除外层死皮层的方法是使用砂纸。酒精的预处理不是强制?...

仔猪是神经科学1的新兴模型系统。为了加强翻译研究, 我们发明了一种从无节制仔猪2 (图 1和图 2) 中记录无侵袭性、临床脑电图的方法。对脑电图记录的翻译使用的两个先决条件, 关于与皮质成熟有关的脑电图模式, 是一种非侵入性的方法, 可与临床设置相媲美, 也可以节制镇静剂或麻醉。单通道遥测系统3与自粘电极结合, 可固定在5分钟左右. 此后, 仔猪将迅速从处理过程中恢复, 并将其喂养和睡眠行为与其他小猪和母猪。
虽然已经有尝试使用非侵入性脑电图记录从镇静动物4, 多数脑电图研究从动物是以侵入的方法进行。这些方法对植入电极5、6周围的炎症过程有副作用, 在大多数情况下, 由于植入脑电图系统的外部成分, 它们需要动物的社会分离。因此, 将这些数据翻译到临床背景是困难的。对翻译方法的需要越来越清楚, 因为它仍然不知道如何在早期皮质发育期间的 "正常" 大脑成熟是由临床, 非侵入性脑电图7表示。这一知识缺口是由8早产儿脑电图记录所带来的技术挑战引起的。在动物模型系统中, 早期皮质发育的模式更容易获得, 因为大多数动物与人类皮质发育相比, 出生时有 "早产儿"9。除了2种动物皮质发育的保守模式外, 最近还发现早产儿的脑电图记录也可以预测10、11以后的个体临床结局。这里描述的方法对于发展神经科学的转化方面特别有用。

<table border="0" cellpadding="0" cellspacing="0" width="867">
	<tbody>
		<tr height="63">
			<td height="63" style="height:63px;width:216px;">Disposable adhesive 
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			Medica S.r.l., Genova, Italy</td>
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			<td style="width:223px;">Self adhesive hydrogel electrode</td>
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		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Abralyt HiCl</td>
			<td style="width:309px;">Easycap GmbH</td>
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			<td>Abrasive cream</td>
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			<td>Skin adhesive silicone</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Telemetry system</td>
			<td style="width:309px;"></td>
			<td style="width:119px;"></td>
			<td>Internal development</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Picolog 1216</td>
			<td style="width:309px;">Pico Technology</td>
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			<td>AD converter</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Laptop</td>
			<td style="width:309px;">Panasonic</td>
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			<td>Rugged laptop</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Receiver</td>
			<td style="width:309px;"></td>
			<td style="width:119px;"></td>
			<td>Internal development</td>
		</tr>
	</tbody>
</table>

noninvasive eeg recordings from freely moving piglets

该方法允许在猪圈中直接从自由移动的仔猪中记录高质量的脑电图 (脑电图)。采用单通道遥测脑电图系统与标准自粘水凝胶电极结合使用。小猪在没有镇静剂的情况下平静下来。在释放到猪圈后, 小猪的行为正常--他们在同兄弟姐妹一样的循环中喝酒睡觉。他们的睡眠阶段用于脑电图录音。

我们能够记录与非 REM 睡眠相关的典型脑电图模式, 如主轴爆裂或三角洲刷子, 从自由移动的仔猪 (图 1和图 2)。我们主要对非 REM 睡眠期间的代表性模式感兴趣, 但也记录了一个非常低振幅的 REM 睡眠12的阶段 (图 3)。13种动物的生理和 REM 睡眠量各不相同。短 REM 阶段在几?...

Watch this Scientific Journal Video about Noninvasive EEG Recordings from Freely Moving Piglets at JoVE.com

Noninvasive EEG Recordings from Freely Moving Piglets

在这里, 我们提出了一个协议, 记录遥测脑电图 (脑电图) 从自由移动仔猪直接在猪圈没有使用镇静剂, 使其能够记录典型的脑电图模式在非 REM 睡眠, 如纺锤爆发。

自由移动仔猪的无创脑电图记录

The method allows the recording of high-quality electroencephalograms (EEGs) from freely moving piglets directly in the pigpen. We use a one-channel ...

Research

JoVE Journal

Neuroscience

High-density EEG Recordings of the Freely Moving Mice using Polyimide-based Microelectrode

高密度的自由移动，使用基于聚酰亚胺的微电极的小鼠的脑电图记录

Electroencephalogram (EEG) indicates the averaged electrical activity of the neuronal populations on a large-scale level. It is widely utilized as a ...

科研

神经科学

Recording Single Neurons' Action Potentials from Freely Moving Pigeons Across Three Stages of Learning

自由移动鸽子穿越三个阶段学习记录单个神经元&#39;动作电位

While the subject of learning has attracted immense interest from both behavioral and neural scientists, only relatively few investigators have observed ...

A Novel Behavioral Assay to Investigate Gustatory Responses of Individual, Freely-moving Bumble Bees (Bombus terrestris)

A Novel Behavioral Assay to Investigate Gustatory Responses of Individual, Freely-moving Bumble Bees Bombus terrestris

一种新的行为分析调查个人的味觉，可自由移动的熊蜂（<em&gt;欧洲熊蜂</em&gt;）

Generalist pollinators like the buff-tailed bumble bee, Bombus terrestris, encounter both nutrients and toxins in the floral nectar they collect from ...

Recording EEG in Freely Moving Neonatal Rats Using a Novel Method

使用新颖的方法在自由移动的新生大鼠中记录脑电图

EEG is a useful method to detect electrical activity in the brain. Moreover, it is a widely used diagnostic tool for various neurological conditions, such ...

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

自由运动大鼠皮质局部电位、心电图、肌电图和呼吸节律的同步记录

Monitoring the physiological dynamics of the brain and peripheral tissues is necessary for addressing a number of questions about how the brain controls ...

Microdialysis of Excitatory Amino Acids During EEG Recordings in Freely Moving Rats

自由运动大鼠脑电图记录过程中兴奋氨基酸的微透析

Microdialysis is a well-established neuroscience technique that correlates the changes of neurologically active substances diffusing into the brain ...

Multi-Fiber Photometry to Record Neural Activity in Freely-Moving Animals

多纤维光测量记录自由移动动物的神经活动

Recording the activity of a group of neurons in a freely-moving animal is a challenging undertaking. Moreover, as the brain is dissected into smaller and ...

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

自由移动小鼠中肠道来源的微生物代谢物的脑室内递送

The impact of gut microbiota and their metabolites on host physiology and behavior has been extensively investigated in this decade. Numerous studies have ...

Extracellular Electrophysiological Recording in the Motor Cortex of Mice

Multichannel Extracellular Recording in Freely Moving Mice

Advancements in Multichannel Extracellular Recording for Studying Neuronal Activity in Freely Moving Mice (Video) | JoVE

自由移动小鼠的多通道细胞外记录

The protocol aims to uncover the properties of neuronal firing and network local field potentials (LFPs) in behaving mice carrying out specific tasks by ...

Cerebrospinal Fluid and Blood Withdrawal from Rats with Concurrent EEG Recording

Sampling Cerebrospinal Fluid and Blood from Lateral Tail Vein in Rats During EEG Recordings

Obtaining High-Quality CSF and Blood Samples for Epilepsy Biomarker Discovery (Video) | JoVE

在脑电图记录期间从大鼠的侧尾静脉采集脑脊液和血液

Because the composition of body fluids reflects many physiological and pathological dynamics, biological liquid samples are commonly obtained in many ...