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DOI :
10.3791/58228-v
January 7th, 2019
Chapters
0:04
Title
0:30
Agarose Salt Bridges Between the Bath Solution and Reference Electrode
1:29
Application of Fluid Flow Shear Force to Cells in a Patch-Clamping Chamber
2:21
Measuring Changes in Liquid-Metal Junction Potential
3:18
Experimental Estimation of Real Chloride Concentration
4:07
Results: Effects of Fluid Flow on L-type VDCCL and Kir2.1 Currents With and Without Agarose 3 M KCl Bridge
5:14
Conclusion
利用膜片钳记录, 从流体流动剪切力敏感性的角度研究了机械敏感离子通道。然而, 根据实验方案, 离子通道流体流动规律的结果可能是错误的。在这里, 我们提供了防止和纠正此类错误的方法, 并提供了理论依据。
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