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DOI :
10.3791/58267-v
September 1st, 2018
Chapters
0:04
Title
0:28
Phage Lysate Preparation
2:31
P1 Transduction
4:30
Kanamycin (Kan) Cassette Excision
6:03
Results: TAA Expression by a tamA Deletion Strain
7:38
Conclusion
在这里, 我们提出了使用预先存在的抗生素耐药性-盒式删除结构作为一个基础, 使删除突变体在其他大肠杆菌菌株。这种删除突变可以调动和插入到相应的受体菌株的位置使用 P1 噬菌体转导。
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