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DOI :
10.3791/58270-v
December 4th, 2018
Chapters
0:04
Title
0:41
Fixation, Cell Lysis, and Chromatin Shearing
3:54
Chromatin Immunoprecipitation
8:31
Results: qRT-PCR with Precipitated DNA Identified Specific Binding of NFAT2 to the LCK Promotor Region
9:30
Conclusion
慢性淋巴细胞白血病 (cll) 是西方世界最常见的白血病。nfat 转录因子是多种细胞类型发育和激活的重要调节剂。在这里, 我们提出了一个在人的 cll 细胞中使用染色质免疫沉淀 (chip) 来识别 nfat2 的新靶向基因的协议。
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