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DOI :
10.3791/58279-v
October 24th, 2018
Chapters
0:04
Title
0:41
Step I: Corneal Induction in Suspension Culture
3:07
Step II: Corneal Differentiation in Adherent Culture
4:33
Step III: Cryo-banking Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
6:08
Step IV: Thawing the Cryopreserved Human Pluripotent Stem Cell-derived Limbal Epithelial Stem Cells
7:25
Results: Differentiation of Human Pluripotent Stem Cells to Clinically Compatible Corneal Limbal Epithelial Stem Cells
9:31
Conclusion
このプロトコルは、xeno ・ フィーダー細胞フリー培養条件下でのひと多能性幹細胞から角膜輪部上皮幹細胞の差別化のための単純な 2 段階法を紹介します。ここで示した細胞培養法は、医療の質細胞、角膜上皮細胞療法の使用に適用される低コスト大量生産を有効にします。
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