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DOI :
10.3791/58308-v
August 25th, 2018
Chapters
0:04
Title
0:36
Immunofluorescence Staining of the Paraffin-embedded Epididymal Sections
2:44
Isolation of Epididymosomes from the Mouse Caput Epididymis
5:26
Immunofluorescence Staining of mECap18 Cells
6:22
Isolation of Proteins from Conditioned Cell Culture Medium
8:06
Results: Analysis of Epididymal Protein Synthesis and Secretion
9:45
Conclusion
在这里, 我们报告动力蛋白的免疫荧光定位, 以说明在石蜡嵌入小鼠附睾切片和永生化附睾细胞系 (mECap18) 的蛋白质检测协议。我们还描述了从附睾液和条件细胞培养基中分离分泌蛋白的协议。
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