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DOI :
10.3791/58308-v
August 25th, 2018
Chapters
0:04
Title
0:36
Immunofluorescence Staining of the Paraffin-embedded Epididymal Sections
2:44
Isolation of Epididymosomes from the Mouse Caput Epididymis
5:26
Immunofluorescence Staining of mECap18 Cells
6:22
Isolation of Proteins from Conditioned Cell Culture Medium
8:06
Results: Analysis of Epididymal Protein Synthesis and Secretion
9:45
Conclusion
パラフィン埋め込まれたマウス精巣のセクションで蛋白質の検出と精巣細胞の不死化線 (mECap18) のためのプロトコルを説明するダイナミンの蛍光抗体法により局在を報告する.また精巣上体液やエアコン携帯メディアから分泌蛋白質の隔離のためのプロトコルについて述べる。
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