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A Cell-based Assay to Investigate Non-muscle Myosin II Contractility via the Folded-gastrulation Signaling Pathway in Drosophila S2R+ Cells

DOI :

10.3791/58325-v

7:15 min

August 19th, 2018

August 19th, 2018

7,085 Views

1Department of Biology, The University of North Carolina at Chapel Hill, 2Department of Biology, Reed College, 3Carolina Center for Genome Sciences, The University of North Carolina at Chapel Hill, 4Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill

Here we describe a contractility assay using Drosophila S2R+ cells. The application of an exogenous ligand, folded gastrulation (Fog), leads to the activation of the Fog signaling pathway and cellular contractility. This assay can be used to investigate the regulation of cellular contractility proteins in the Fog signaling pathway.

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Cell based Assay

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