A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

16.7K Views

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10:07 min

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May 17th, 2017

DOI :

10.3791/55565-v

May 17th, 2017

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Zhaofeng Jia¹^,², Yujie Liang³, Bin Ma⁴^,⁵, Xiao Xu²^,⁶, Jianyi Xiong², Li Duan², Daping Wang¹^,²

¹Guangzhou Medical University, ²Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), ³Department of Chemistry, The Chinese University of Hong Kong, ⁴School of Biomedical Engineering, Shanghai Jiao Tong University, ⁵Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, ⁶Shantou University Medical College

Summary

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Keywords 5 mC Dot Blot

DNA Methylation

Chondrocyte Dedifferentiation

Autologous Chondrocyte Implantation

Chapters in this video

0:05

Title

1:52

Human Articular Cartilage Tissue Collection and Chondrocyte Culture

3:33

Genomic DNA Extraction

5:47

Dot Blot nalysis

7:55

Results: DNA Methylation Increases During Chondrocyte Dedifferentiation

8:45

Conclusion

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